硫酸甘露聚糖丙二醇酯对兔晶状体上皮细胞体外生长的抑制作用

Inhibitory effect of propylene glycol mannate sulfate on growth of rabbit lens epithelial cells in vitro.

作者信息

Huang Jin, Xie Li-Na

机构信息

Department of Ophthalmology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing 210006, Jiangsu Province, China.

出版信息

Int J Ophthalmol. 2010;3(1):54-7. doi: 10.3980/j.issn.2222-3959.2010.01.12. Epub 2010 Mar 18.

DOI:10.3980/j.issn.2222-3959.2010.01.12

PMID:22553517

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3340644/

Abstract

AIM

To investigate the inhibitory effect of rabbit lens epithelial cell (RLEC) survival and growth by propylene glycol mannate sulfate (PGMS) on the rabbit capsular bag in vitro.

METHODS

Capsular bags were prepared from rabbit eyes after extracapsular cataract extraction (ECCE) and incubated in 0.2, 0.4, 0.8g/L PGMS in 2, 5, 10 minutes incubation periods. After treatment, the capsular bags were cultured for 7 days in Dulbecco minimum essential medium (DMEM) supplemented with 50mL/L fetal calf serum (FCS). The specimens were examined with light microscopy and transmission electron microscopy (TEM). Capsular bags without receiving PGMS only served as controls.

RESULTS

PGMS inhibited the proliferation of RLEC in the manner of concentration and time dependent. At the threshold protocol of incubation in PGMS at 0.8g/L for 5 or 10 minutes, proliferative activity of cells were largely arrested and nearly no RLEC was seen on the posterior capsule (P<0.05). Control group had no effect on structure and proliferative activity of RLEC, and the growth proceeded rapidly so that the posterior capsule were totally covered by a confluent monolayer of cell by the end of 7 days. Under TEM, the cells in the control group were tightly arrayed with clearly defined cellular boundary and structure; while cellular deformity and undefined intracellular structure could be seen in the 0.4g/L and 0.8g/L experimental groups.

CONCLUSION

PGMS can effectively inhibit the proliferation of RLEC.

摘要

目的

研究硫酸甘露聚糖丙二醇酯（PGMS）对兔晶状体囊袋内兔晶状体上皮细胞（RLEC）存活和生长的抑制作用。

方法

在兔行白内障囊外摘除术（ECCE）后制备囊袋，并分别在浓度为0.2、0.4、0.8g/L的PGMS中孵育2、5、10分钟。处理后，将囊袋在补充有50mL/L胎牛血清（FCS）的杜尔贝科改良 Eagle 培养基（DMEM）中培养7天。用光学显微镜和透射电子显微镜（TEM）检查标本。未接受PGMS处理的囊袋仅作为对照。

结果

PGMS以浓度和时间依赖性方式抑制RLEC的增殖。在PGMS浓度为0.8g/L孵育5或10分钟的阈值方案下，细胞增殖活性被大大抑制，后囊膜上几乎未见RLEC（P<0.05）。对照组对RLEC的结构和增殖活性无影响，生长迅速，到7天结束时后囊膜完全被汇合的单层细胞覆盖。在TEM下，对照组细胞排列紧密，细胞边界和结构清晰；而在0.4g/L和0.8g/L实验组中可见细胞变形和细胞内结构不明确。