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葛根素对过氧亚硝酸盐通过Fas/FasL途径诱导的视网膜色素上皮细胞凋亡的影响。

Effect of puerarin on retinal pigment epithelial cells apoptosis induced partly by peroxynitrite via Fas/FasL pathway.

作者信息

Hao Li-Na, Zhang Yan-Qing, Shen Yu-Hua, Wang Zhi-Yun, Wang Yan-Hua, Zhang Hai-Fang, He Shou-Zhi

机构信息

Department of Ophthalmology, People's Hospital of Hebei Province, Shijiazhuang 050051, Hebei Province, China.

出版信息

Int J Ophthalmol. 2010;3(4):283-7. doi: 10.3980/j.issn.2222-3959.2010.04.01. Epub 2010 Dec 18.

DOI:10.3980/j.issn.2222-3959.2010.04.01
PMID:22553574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3340750/
Abstract

AIM

To evaluate the peroxynitrite (ONOO(-)) of puerarin on retinal pigment epithelial (RPE) cells apoptosis induced partly by peroxynitrite via Fas/FasL.

METHODS

RPE cells from C57BL/6 mice eyes were cultured. Diabetes was induced in Sprague-Dawley (SD) rats by streptozotocin (STZ) intraperitoneal injection. Puerarin was administrated to cultured RPE cells and diabetic rats. Western blotting analysis, DNA ladder, RT-PCR, immunohistochemistry were used for determining the expression of nitrotyrosine (NT, the foot print of ONOO(-)), complement 3 (C3); apoptosis and inducible nitric oxide synthase (iNOS) mRNA as well as Fas/FasL signal transduction in RPE cells.

RESULTS

Both RPE cells in ONOO(-) and puerarin group developed apoptosis and expressed NT, C3, iNOS mRNA and Fas/FasL. But latter delayed the all changes in a time-dependent manner compared with control and STZ group (P<0.001). iNOS, C3 and Fas/FasL were up-regulated and associated with an increase of expression of ONOO(-)in vivo and in vitro.

CONCLUSION

Puerarin decreases RPE cells apoptosis partly induced by ONOO(-) for diabetic retinopathy.

摘要

目的

评估葛根素对过氧亚硝酸根（ONOO⁻）通过Fas/FasL途径部分诱导的视网膜色素上皮（RPE）细胞凋亡的影响。

方法

培养C57BL/6小鼠眼的RPE细胞。通过腹腔注射链脲佐菌素（STZ）诱导Sprague-Dawley（SD）大鼠患糖尿病。将葛根素应用于培养的RPE细胞和糖尿病大鼠。采用蛋白质免疫印迹分析、DNA梯状条带分析、逆转录聚合酶链反应（RT-PCR）、免疫组织化学等方法，检测硝基酪氨酸（NT，ONOO⁻的标志物）、补体3（C3）的表达；RPE细胞中的细胞凋亡、诱导型一氧化氮合酶（iNOS）mRNA以及Fas/FasL信号转导情况。

结果

ONOO⁻组和葛根素组的RPE细胞均发生凋亡，并表达NT、C3、iNOS mRNA和Fas/FasL。但与对照组和STZ组相比，葛根素组能以时间依赖的方式延缓所有这些变化（P<0.001）。iNOS、C3和Fas/FasL在体内和体外均上调，并与ONOO⁻表达的增加相关。

结论

葛根素可减少过氧亚硝酸根部分诱导的糖尿病视网膜病变RPE细胞凋亡。

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