曲格列酮通过线粒体依赖性途径诱导人翼状胬肉成纤维细胞凋亡。
Troglitazone induced apoptosis of human pterygium fibroblasts through a mitochondrial-dependent pathway.
作者信息
Yang Xiao-Xi, Chen Jian, Zhou Qing, Guo Xian-Yin, Xiao Pan, Wu Jing, Xu Jin-Tang
机构信息
Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China.
出版信息
Int J Ophthalmol. 2011;4(2):143-6. doi: 10.3980/j.issn.2222-3959.2011.02.06. Epub 2011 Apr 18.
AIM
To study the effect of troglitazone on primary culture human pterygium fibroblasts (HPF).
METHODS
Cell viability loss and apoptosis were quantified by cell counting kit-8, AnnexinV-FITC/PI double staining, caspases activity test and western blotting. Flow cytometry was used to detect mitochondrial membrane potential.
RESULTS
Peroxisome proliferator-activated receptor γ (PPAR-γ) was positively expressed in pterygium specimens (n=5). Troglitazone showed dose-dependent inhibition of cell survival, induced phospholipids redistribution, activated caspase-3, -9, and altered mitochondrial potential. Western blot assay demonstrated the increase of Bax/Bcl-2 protein ratio.
CONCLUSION
Troglitazone induced apoptosis of HPF through a mitochondrial-dependent pathway.
目的
研究曲格列酮对原代培养的人翼状胬肉成纤维细胞（HPF）的作用。
方法
采用细胞计数试剂盒-8、膜联蛋白V-异硫氰酸荧光素/碘化丙啶双染色、半胱天冬酶活性检测及蛋白质免疫印迹法对细胞活力丧失和凋亡进行定量分析。运用流式细胞术检测线粒体膜电位。
结果
过氧化物酶体增殖物激活受体γ（PPAR-γ）在翼状胬肉标本（n=5）中呈阳性表达。曲格列酮对细胞存活具有剂量依赖性抑制作用，诱导磷脂重分布，激活半胱天冬酶-3、-9，并改变线粒体电位。蛋白质免疫印迹分析显示Bax/Bcl-2蛋白比率增加。
结论
曲格列酮通过线粒体依赖性途径诱导HPF凋亡。
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