Troglitazone induced apoptosis of human pterygium fibroblasts through a mitochondrial-dependent pathway.
作者信息
Yang Xiao-Xi, Chen Jian, Zhou Qing, Guo Xian-Yin, Xiao Pan, Wu Jing, Xu Jin-Tang
机构信息
Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China.
出版信息
Int J Ophthalmol. 2011;4(2):143-6. doi: 10.3980/j.issn.2222-3959.2011.02.06. Epub 2011 Apr 18.
Abstract
AIM
To study the effect of troglitazone on primary culture human pterygium fibroblasts (HPF).
METHODS
Cell viability loss and apoptosis were quantified by cell counting kit-8, AnnexinV-FITC/PI double staining, caspases activity test and western blotting. Flow cytometry was used to detect mitochondrial membrane potential.
RESULTS
Peroxisome proliferator-activated receptor γ (PPAR-γ) was positively expressed in pterygium specimens (n=5). Troglitazone showed dose-dependent inhibition of cell survival, induced phospholipids redistribution, activated caspase-3, -9, and altered mitochondrial potential. Western blot assay demonstrated the increase of Bax/Bcl-2 protein ratio.
CONCLUSION
Troglitazone induced apoptosis of HPF through a mitochondrial-dependent pathway.
摘要
相似文献
1
Troglitazone induced apoptosis of human pterygium fibroblasts through a mitochondrial-dependent pathway.
Int J Ophthalmol. 2011;4(2):143-6. doi: 10.3980/j.issn.2222-3959.2011.02.06. Epub 2011 Apr 18.
2
Ligands of peroxisome proliferator-activated receptor-gamma induce apoptosis in AR42J cells.
Pancreas. 2002 Mar;24(2):130-8. doi: 10.1097/00006676-200203000-00003.
3
A PPAR-Gamma Agonist Rosiglitazone Suppresses Fibrotic Response in Human Pterygium Fibroblasts by Modulating the p38 MAPK Pathway.
Invest Ophthalmol Vis Sci. 2017 Oct 1;58(12):5217-5226. doi: 10.1167/iovs.17-22203.
4
A peroxisome proliferator-activated receptor-gamma agonist, troglitazone, facilitates caspase-8 and -9 activities by increasing the enzymatic activity of protein-tyrosine phosphatase-1B on human glioma cells.
J Biol Chem. 2006 Mar 10;281(10):6165-74. doi: 10.1074/jbc.M505266200. Epub 2005 Nov 30.
5
Peroxisome proliferator-activated receptor gamma ligand troglitazone induces cell cycle arrest and apoptosis of hepatocellular carcinoma cell lines.
Cancer. 2002 Nov 15;95(10):2243-51. doi: 10.1002/cncr.10906.
6
Troglitazone, a peroxisome proliferator-activated receptor gamma ligand, induces growth inhibition and apoptosis of HepG2 human liver cancer cells.
World J Gastroenterol. 2008 Apr 14;14(14):2168-73. doi: 10.3748/wjg.14.2168.
7
A ligand for peroxisome proliferator activated receptor gamma inhibits cell growth and induces apoptosis in human liver cancer cells.
Gut. 2002 Apr;50(4):563-7. doi: 10.1136/gut.50.4.563.
8
Downregulation of cyclooxygenase-2 expression and activation of caspase-3 are involved in peroxisome proliferator-activated receptor-gamma agonists induced apoptosis in human monocyte leukemia cells in vitro.
Ann Hematol. 2007 Mar;86(3):173-83. doi: 10.1007/s00277-006-0205-2. Epub 2006 Nov 7.
9
Effect of ligand troglitazone on peroxisome proliferator-activated receptor gamma expression and cellular growth in human colon cancer cells.
World J Gastroenterol. 2006 Dec 7;12(45):7263-70. doi: 10.3748/wjg.v12.i45.7263.
10
Suppression of NF-kappaB and GSK-3beta is involved in colon cancer cell growth inhibition by the PPAR agonist troglitazone.
Chem Biol Interact. 2010 Oct 6;188(1):75-85. doi: 10.1016/j.cbi.2010.06.001. Epub 2010 Jun 9.
引用本文的文献
1
Role of Peroxisome Proliferator-Activated Receptor γ in Ocular Diseases.
J Ophthalmol. 2015;2015:275435. doi: 10.1155/2015/275435. Epub 2015 Jun 4.
本文引用的文献
1
Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) ligands as potential therapeutic agents to treat arthritis.
Pharmacol Res. 2009 Sep;60(3):160-9. doi: 10.1016/j.phrs.2009.02.005. Epub 2009 Feb 14.
2
Rosiglitazone and delayed onset of proliferative diabetic retinopathy.
Arch Ophthalmol. 2008 Jun;126(6):793-9. doi: 10.1001/archopht.126.6.793.
3
PPARs in Eye Biology and Disease.
PPAR Res. 2008;2008:518790. doi: 10.1155/2008/518790.
4
Mechanisms of anti-inflammatory and neuroprotective actions of PPAR-gamma agonists.
Front Biosci. 2008 Jan 1;13:1813-26. doi: 10.2741/2802.
5
Effect of overexpression of PPARgamma on the healing process of corneal alkali burn in mice.
Am J Physiol Cell Physiol. 2007 Jul;293(1):C75-86. doi: 10.1152/ajpcell.00332.2006.
6
UVB-mediated induction of cytokines and growth factors in pterygium epithelial cells involves cell surface receptors and intracellular signaling.
Invest Ophthalmol Vis Sci. 2006 Jun;47(6):2430-7. doi: 10.1167/iovs.05-1130.
7
Fibroblasts isolated from human pterygia exhibit altered lipid metabolism characteristics.
Exp Eye Res. 2006 Sep;83(3):536-42. doi: 10.1016/j.exer.2006.02.005. Epub 2006 May 11.
8
Investigation of nitric oxide in pterygium.
Can J Ophthalmol. 2005 Dec;40(6):743-6. doi: 10.1016/S0008-4182(05)80092-4.
9
Receptor-independent actions of PPAR thiazolidinedione agonists: is mitochondrial function the key?
Biochem Pharmacol. 2005 Jul 15;70(2):177-88. doi: 10.1016/j.bcp.2005.03.033.
10
Detection of point mutations at codon 12 of KI-ras in ophthalmic pterygia.
Eye (Lond). 2005 Feb;19(2):210-4. doi: 10.1038/sj.eye.6701452.
Zotero 插件