Wang Yun, Tan Juan, Zhuo Ye-Hong, Wang Cui-Lan, Liu Xu-Yang, Cai Su-Ping
Ophthalmic Laboratories & Department of Ophthalmology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Int J Ophthalmol. 2011;4(3):239-42. doi: 10.3980/j.issn.2222-3959.2011.03.04. Epub 2011 Jun 18.
To determine the effects of a low dose latrunculin (LAT)-A on dexamethasone (Dex)-induced upregulation of extracellular matrix proteins fibronectin (FN) in cultured human trabecular meshwork (HTM) cells.
HTM cells were cultured to confluent and incubated with 0.4µmol/L Dex and/or 0.05µmol/L LAT-A. FN expression in HTM cells was evaluated by Western blot and immunofluorescence microscopy.
Dex up-regulated FN production in HTM cells, failed to do so when co-incubated with LAT-A. LAT-A decreased production of FN in cultured HTM cells.
This study indicated that LAT-A may modulate the expression of fibronectin in trabecular meshwork to achieve treatment for steroids and other types of glaucoma. It has an important prospect as an intraocular pressure- lowering drug.
确定低剂量拉春库林（LAT）-A对培养的人小梁网（HTM）细胞中地塞米松（Dex）诱导的细胞外基质蛋白纤连蛋白（FN）上调的影响。
将HTM细胞培养至汇合状态，并用0.4µmol/L Dex和/或0.05µmol/L LAT-A孵育。通过蛋白质免疫印迹法和免疫荧光显微镜评估HTM细胞中FN的表达。
Dex上调了HTM细胞中FN的产生，当与LAT-A共同孵育时则未能上调。LAT-A降低了培养的HTM细胞中FN的产生。
本研究表明，LAT-A可能调节小梁网中纤连蛋白的表达，以实现对类固醇性和其他类型青光眼的治疗。作为一种降眼压药物，它具有重要的应用前景。
