慢性高眼压大鼠视网膜神经节细胞中的转化生长因子-β(1)：其表达及抗凋亡作用
TGF-β(1) in retinal ganglion cells in rats with chronic ocular hypertension: its expression and anti-apoptotic effect.
作者信息
Tao Yong-Jian, Gao Dian-Wen, Yu Miao
机构信息
Department of Ophthalmology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.
出版信息
Int J Ophthalmol. 2011;4(4):396-401. doi: 10.3980/j.issn.2222-3959.2011.04.15. Epub 2011 Aug 18.
AIM
To investigate the anti-apoptotic effect of transforming growth factor beta-1 (TGF-β(1)) on chronic ocular hypertension.
METHODS
The expression of TGF-β(1) in retinal ganglion cells (RCGs) was measured using the immunohistochemiscal S-P method and real-time PCR in the normally control group, the ocular hypertension group (experimental group A), the ocular hypertension plus antibody intervention group (experimental group B) and the ocular hypertension plus antigen intervention group (experimental group C) at 1, 2, 3 and 4 weeks postoperatively. The count of apoptotic RCGs was measured using the TUNEL method.
RESULTS
The expression of TGF-β(1 )was significantly higher in experimental group C than that in other three groups (P<0.05). The expression was the lowest in experimental group B (4.17%). A statistically significant difference was noted between the four groups (P<0.01). The count of apoptotic RCGs was statistically significantly lower in experimental group C than that in the experimental groups A and B (P<0.01). A statistically significant difference was noted in the count of apoptotic RCGs between these three experimental groups (P<0.01).
CONCLUSION
TGF-β(1) can inhibit the apoptosis of RCGs in rats with chronic ocular hypertension.
目的
研究转化生长因子β-1（TGF-β(1)）对慢性高眼压的抗凋亡作用。
方法
采用免疫组化S-P法和实时定量PCR检测正常对照组、高眼压组（实验组A）、高眼压加抗体干预组（实验组B）和高眼压加抗原干预组（实验组C）术后1、2、3和4周视网膜神经节细胞（RGCs）中TGF-β(1)的表达。采用TUNEL法检测凋亡RGCs的数量。
结果
实验组C中TGF-β(1)的表达明显高于其他三组（P<0.05）。实验组B中表达最低（4.17%）。四组之间差异有统计学意义（P<0.01）。实验组C中凋亡RGCs的数量明显低于实验组A和B（P<0.01）。这三个实验组之间凋亡RGCs数量差异有统计学意义（P<0.01）。
结论
TGF-β(1)可抑制慢性高眼压大鼠RGCs的凋亡。
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