College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Biochem Biophys Res Commun. 2012 May 18;421(4):797-800. doi: 10.1016/j.bbrc.2012.04.089. Epub 2012 Apr 25.
Survivin is a member of inhibitors of apoptosis proteins (IAPs), and also belongs to be a member of the chromosomal passenger complex (CPC) which has multiple functions including inhibition of apoptosis and regulation of cell division and SAC activity. Plk1 (polo-like kinase 1) associates with the spindle poles and also distributes to the kinetochores and is shown to involve in spindle organization, APC/C activation and cytokinesis in many models. Our recent work has shown that Survivin is a critical regulator of chromosome segregation and spindle assembly checkpoint (SAC) in meiosis. In the present study, we found that Plk1 co-localized with Survivin at metaphase I (MI) and telophase I (TI) stage after GVBD. Plk1 dispersed into the oocyte cytoplasm or accumulated near the chromosomes after the depletion of Survivin by morpholino (MO) injection. Our results showed that the localization of Plk1 to kinetochores required the involvement of Survivin.
Survivin 是凋亡抑制蛋白（IAPs）的成员，也属于染色体乘客复合物（CPC）的成员，具有多种功能，包括抑制细胞凋亡和调节细胞分裂和 SAC 活性。Plk1（polo 样激酶 1）与纺锤体极结合，并分布到动粒上，在许多模型中被证明参与纺锤体组织、APC/C 激活和胞质分裂。我们最近的工作表明，Survivin 是减数分裂中染色体分离和纺锤体组装检查点（SAC）的关键调节因子。在本研究中，我们发现 Plk1 在 GVBD 后与 Survivin 在中期 I（MI）和末期 I（TI）阶段共定位。用 Survivin 的 morpholino（MO）注射耗尽后，Plk1 分散到卵母细胞细胞质中或聚集在染色体附近。我们的结果表明，Plk1 向动粒的定位需要 Survivin 的参与。
