Discipline of Anatomy and Pathology, The University of Adelaide, North Terrace, Adelaide, South Australia 5005, Australia.
Acta Biomater. 2012 Aug;8(8):3104-12. doi: 10.1016/j.actbio.2012.04.037. Epub 2012 Apr 30.
Wear particle-induced orthopaedic prosthesis loosening is associated with elevated osteoclast activity. The immunoreceptor tyrosine-based activation motif (ITAM)-related molecules OSCAR, FcRγ, TREM2 and DAP12 are important for osteoclast formation. The aim of this study was to determine if these molecules are involved in peri-implant loosening by investigating their expression in peri-implant tissues obtained at revision of joint replacement components containing polyethylene (PE) wear particles, and in osteoclasts formed in vitro in the presence of PE particles. The results showed that there was a marked and statistically significant increase in protein levels of the ITAM-related molecules in the revision tissues. The levels of OSCAR, FcRγ, TREM2 and DAP12 mRNA in the revision tissues were also increased. In vitro PE particles stimulated osteoclast resorption in the presence of 50 ng ml(-1) receptor activator NFκB (RANKL) and significantly elevated the expression of OSCAR, FcRγ, TREM2 and DAP12 during osteoclast formation. These findings suggest that the ITAM signalling molecules and their co-receptors have a role in pathogenic bone loss associated with implant PE wear.
磨屑诱导的骨科假体松动与破骨细胞活性升高有关。免疫受体酪氨酸激活基序（ITAM）相关分子 OSCAR、FcRγ、TREM2 和 DAP12 对破骨细胞形成很重要。本研究旨在通过研究从含聚乙烯（PE）磨屑的关节置换部件翻修中获得的植入物周围组织以及在存在 PE 颗粒的情况下体外形成的破骨细胞中这些分子的表达，来确定它们是否参与植入物周围松动。结果表明，在翻修组织中 ITAM 相关分子的蛋白水平显著且具有统计学意义的增加。翻修组织中 OSCAR、FcRγ、TREM2 和 DAP12 的 mRNA 水平也增加。体外 PE 颗粒在 50ng/ml 核因子 kappa B 受体激活剂（RANKL）存在下刺激破骨细胞吸收，并在破骨细胞形成过程中显著上调 OSCAR、FcRγ、TREM2 和 DAP12 的表达。这些发现表明，ITAM 信号分子及其共受体在与植入物 PE 磨损相关的致病性骨丢失中起作用。
