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对需氧异养细菌进行检测无法预测牙科治疗椅单元输出水中是否存在潜在致病细菌污染。

Testing for aerobic heterotrophic bacteria allows no prediction of contamination with potentially pathogenic bacteria in the output water of dental chair units.

作者信息

Bristela Margit, Skolka Astrid, Schmid-Schwap Martina, Piehslinger Eva, Indra Alexander, Wewalka Günther, Stauffer Fritz

机构信息

Bernhard Gottlieb University Clinic of Dentistry, Department of Fixed and Removable Prosthodontics, Medical University Vienna, Austria.

出版信息

GMS Krankenhhyg Interdiszip. 2012;7(1):Doc12. doi: 10.3205/dgkh000196. Epub 2012 Apr 4.

DOI:10.3205/dgkh000196
PMID:22558046
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3334951/
Abstract

BACKGROUND

Currently, to our knowledge, quality of output water of dental chair units is not covered by specific regulations in the European Union, and national recommendations are heterogeneous. In Germany, water used in dental chair units must follow drinking water quality. In the United States of America, testing for aerobic heterotrophic bacteria is recommended. The present study was performed to evaluate whether the counts of aerobic heterotrophic bacteria correlate with the presence of potentially pathogenic bacteria such as Legionella spp. or Pseudomonas aeruginosa.

METHODS

71 samples were collected from 26 dental chair units with integrated disinfection device and 31 samples from 15 outlets of the water distribution pipework within the department were examined. Samples were tested for aerobic heterotrophic bacteria at 35°C and 22°C using different culture media and for Legionella spp. and for Pseudomonas aeruginosa. Additionally, strains of Legionella pneumophila serogroup 1 were typed with monoclonal antibodies and representative samples of Legionella pneumophila serogroup 1 were typed by sequence based typing.

RESULTS

Our results showed a correlation between different agars for aerobic heterotrophic bacteria but no correlation for the count of aerobic heterotrophic bacteria and the presence of Legionella spp. or Pseudomonas aeruginosa.

CONCLUSION

Testing for aerobic heterotrophic bacteria in output water or water distribution pipework within the departments alone is without any value for predicting whether the water is contaminated with potentially pathogenic bacteria like Legionella spp. or Pseudomonas aeruginosa.

摘要

背景

据我们所知,目前欧盟尚无关于牙科治疗椅单元产出水质量的具体法规,各国的建议也不尽相同。在德国,牙科治疗椅单元使用的水必须符合饮用水质量标准。在美国,建议对需氧异养细菌进行检测。本研究旨在评估需氧异养细菌的数量是否与嗜肺军团菌或铜绿假单胞菌等潜在致病细菌的存在相关。

方法

从26个配备集成消毒装置的牙科治疗椅单元采集了71份样本,并对科室配水管网的15个出水口采集的31份样本进行了检测。使用不同培养基在35°C和22°C下对样本进行需氧异养细菌检测,以及对嗜肺军团菌和铜绿假单胞菌进行检测。此外,用单克隆抗体对嗜肺军团菌血清1型菌株进行分型,并用基于序列的分型方法对嗜肺军团菌血清1型的代表性样本进行分型。

结果

我们的结果显示,用于检测需氧异养细菌的不同琼脂之间存在相关性,但需氧异养细菌的数量与嗜肺军团菌或铜绿假单胞菌的存在之间没有相关性。

结论

仅对牙科治疗椅单元产出水或科室配水管网中的需氧异养细菌进行检测,对于预测水是否被嗜肺军团菌或铜绿假单胞菌等潜在致病细菌污染没有任何价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/54f8559ea4ca/KHI-07-12-g-005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/e114fa282229/KHI-07-12-g-001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/08a342f4b56a/KHI-07-12-g-002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/f85e6beef920/KHI-07-12-g-003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/20dc7d94bc83/KHI-07-12-g-004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/54f8559ea4ca/KHI-07-12-g-005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/e114fa282229/KHI-07-12-g-001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/08a342f4b56a/KHI-07-12-g-002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/f85e6beef920/KHI-07-12-g-003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/20dc7d94bc83/KHI-07-12-g-004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4de1/3334951/54f8559ea4ca/KHI-07-12-g-005.jpg

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Do contaminated dental unit waterlines pose a risk of infection?受污染的牙科设备水线会带来感染风险吗?
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A novel automated waterline cleaning system that facilitates effective and consistent control of microbial biofilm contamination of dental chair unit waterlines: a one-year study.
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Isolation of pathogenic Legionella species and legionella-laden amoebae in dental unit waterlines.牙科设备水管中致病性军团菌属及携带军团菌的变形虫的分离
J Hosp Infect. 2005 Nov;61(3):257-62. doi: 10.1016/j.jhin.2005.05.001. Epub 2005 Aug 15.
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Microbiological evaluation of dental unit water systems in general dental practice in Europe.欧洲普通牙科诊所牙科设备水系统的微生物学评估。
Eur J Oral Sci. 2004 Oct;112(5):412-8. doi: 10.1111/j.1600-0722.2004.00151.x.
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