Choi Choong-Ho, Ha Myung-Ok, Youn Hye-Jeong, Jeong Seong-Soog, Iijima Youichi, Sohn Woosung, Hong Suk-Jin
Department of Preventive and Public Health Dentistry, Dental Science Research Institute, Chonnam National University, School of Dentistry, Gwangju, Republic of Korea.
Am J Dent. 2012 Feb;25(1):9-12.
To evaluate the laboratory remineralization effects of a dentifrice with bamboo salt and NaF on artificial caries-like enamel lesions, at both the surface and deep areas.
Early dental caries lesions were formed by treating bovine enamel samples for 48 hours at 37 degrees C with a demineralization solution (pH 5.0) containing 0.1 M lactic acid, 0.2% Carbopol 907, and 50% saturated calcium phosphate tribasic. pH cycling was then performed by immersing the samples in dentifrice slurry for 2 minutes every 8 hours per day, and in demineralization solution for 4 hours and mixed saliva for the remaining time period. The mixed saliva consisted of 50% human saliva and 50% artificial saliva. The surface hardness and the level of mineral surface alterations were analyzed using a hardness tester and transversal microradiography, among negative control (fluoride free), positive control (sodium fluoride 1100 ppm, Crest Cavity Protection), and test dentifrice (3.0% bamboo salt with sodium fluoride 1,000 ppm) groups.
Test and positive control groups significantly increased the level of the surface hardness and decreased mineral loss of the artificial caries-like enamel lesions compared to the negative control (P<0.05). The test dentifrice also significantly decreased the lesion depth compared to the other two groups (P<0.05).
评估含竹盐和氟化钠的牙膏对人工龋样釉质病变表面和深层区域的实验室再矿化效果。
通过将牛牙釉质样本在37℃下用含有0.1M乳酸、0.2%卡波姆907和50%饱和磷酸三钙的脱矿溶液处理48小时来形成早期龋齿病变。然后进行pH循环,方法是每天每8小时将样本浸入牙膏浆液中2分钟,再浸入脱矿溶液中4小时,其余时间浸入混合唾液中。混合唾液由50%的人唾液和50%的人工唾液组成。在阴性对照(无氟)、阳性对照(含1100ppm氟化钠的佳洁士防蛀牙膏)和测试牙膏(含3.0%竹盐和1000ppm氟化钠)组中,使用硬度测试仪和横向显微放射照相术分析表面硬度和矿物表面改变程度。
与阴性对照相比,测试组和阳性对照组显著提高了人工龋样釉质病变的表面硬度水平并减少了矿物质流失(P<0.05)。与其他两组相比,测试牙膏还显著降低了病变深度(P<0.05)。