胃肠道癌症患者的氧化 DNA 损伤增加和抗氧化防御系统受损。

Increased oxidative DNA damage and impaired antioxidant defense system in patients with gastrointestinal cancer.

机构信息

Department of Biochemistry, Faculty of Pharmacy, Gazi University, Ankara, Turkey.

出版信息

Eur J Intern Med. 2012 Jun;23(4):350-4. doi: 10.1016/j.ejim.2012.02.001. Epub 2012 Mar 8.

DOI:10.1016/j.ejim.2012.02.001

PMID:22560384

Abstract

BACKGROUND

Highly active intermediates are formed in aerobic metabolism which in turn leads to cellular damage. It is increasingly proposed that free radicals play a key role in human cancer development. The aim of this study was to investigate changes in 8-hydroxy-deoxyguanosine, nitrite+nitrate, total glutathione, total antioxidant capacity levels and superoxide dismutase, catalase, glutathione peroxidase activities in operative patients with gastrointestinal cancer before and after surgery and compare with inoperative patients.

METHODS

Oxidative stress parameters were measured in 59 gastrointestinal cancer patients and 20 controls. 8-hydroxy-deoxyguanosine was quantitated by Elisa method. Superoxide dismutase, catalase, glutathione peroxidase were assayed with colorimetric methods; Nitrite+nitrate, total glutathione and total antioxidant capacity were assayed with spectrophotometric methods.

RESULTS

8-hydroxy-deoxyguanosine levels in cancer patients were higher than those of control group (p<0.01). Similarly, glutathione levels were increased compared with controls (p<0.01). However, nitrite+nitrate, total antioxidant capacity levels and superoxide dismutase and catalase activities were decreased in cancer patients compared with controls (p<0.01, p<0.01, p<0.05, p<0.01, respectively). The patients were divided into two groups; operative (n = 30) and inoperative (n = 29). A significant difference was found in inoperative group compared with postoperative group according to glutathione peroxidase activity (p<0.05).

DISCUSSION

Our results demonstrate that the oxidant/antioxidant balance was altered in favor of free radicals and DNA damage in gastrointestinal cancer patients. Significant increases in 8-hydroxy-deoxyguanosine, glutathione and decreases in nitrite+nitrate, SOD, CAT activities and antioxidant molecules suggest the possible involvement of oxidative stress in gastrointestinal cancer. Glutathione peroxidase activities in postoperative patients were higher compared to inoperative patients.

摘要

背景

有氧代谢会产生高活性中间产物，从而导致细胞损伤。越来越多的人提出，自由基在人类癌症发展中起关键作用。本研究旨在调查手术前后胃肠道癌症患者与非手术患者的 8-羟基脱氧鸟苷、亚硝酸盐+硝酸盐、总谷胱甘肽、总抗氧化能力水平和超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性的变化，并将其与非手术患者进行比较。

方法

测量了 59 例胃肠道癌症患者和 20 例对照者的氧化应激参数。采用 ELISA 法测定 8-羟基脱氧鸟苷。用比色法测定超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶；用分光光度法测定亚硝酸盐+硝酸盐、总谷胱甘肽和总抗氧化能力。

结果

癌症患者的 8-羟基脱氧鸟苷水平高于对照组（p<0.01）。同样，与对照组相比，谷胱甘肽水平也升高（p<0.01）。然而，与对照组相比，癌症患者的亚硝酸盐+硝酸盐、总抗氧化能力水平和超氧化物歧化酶及过氧化氢酶活性均降低（p<0.01，p<0.01，p<0.05，p<0.01）。将患者分为两组：手术组（n=30）和非手术组（n=29）。与术后组相比，非手术组谷胱甘肽过氧化物酶活性有显著差异（p<0.05）。