Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2012 Jun-Jul;36(1):6-11. doi: 10.1016/j.bios.2012.02.064. Epub 2012 Apr 25.
Interests in using nanoporous metals for biosensing applications have been increasing. Herein, nanotubular mesoporous PdCu (NM-PdCu) alloy is used to fabricate a novel label-free electrochemical immunosensor for cancer biomarker carcinoembryonic antigen (CEA). It operates through physisorption of anti-CEA on NM-PdCu and the mixture of sulfonated graphene sheets (HSO(3)-GS) and thionine (TH) functionalized glassy carbon electrode interface as the detection platform. In this study, chitosan (CS)-PdCu is bound very strongly to carcinoembryonic antibody (anti-CEA), because of the good electron conductivity, high surface area, and good biocompatibility. CS-PdCu is immobilized on electrodes by electrostatic interactions between the negatively charged sulfo group of HSO(3)-GS and the abundant positively charged amino groups of chitosan. TH acts as the redox probe. Under the optimized conditions, the electrochemical immunosensor exhibits a wide working range from 0.01 to 12 ng/mL with a low detection limit of 4.86 pg/mL. The accuracy, reproducibility, and stability of the immunosensor are acceptable. The assay is evaluated for real serum samples, receiving satisfactory results. The nanoporous metal materials-based immunoassay provides a promising approach in clinical application and thus represents a versatile detection method.
人们对纳米多孔金属在生物传感应用中的兴趣日益增加。在此，我们采用管状介孔 PdCu（NM-PdCu）合金，构建了一种新颖的、无需标记的电化学免疫传感器，用于检测癌症生物标志物癌胚抗原（CEA）。该传感器的工作原理是通过 NM-PdCu 对抗 CEA 的物理吸附，以及磺化石墨烯片（HSO(3)-GS）和硫堇（TH）修饰玻碳电极界面混合物作为检测平台。在这项研究中，CS-PdCu 与癌胚抗体（anti-CEA）结合非常牢固，这是因为其具有良好的电子导电性、高表面积和良好的生物相容性。CS-PdCu 通过 HSO(3)-GS 的负电荷与壳聚糖的丰富正电荷之间的静电相互作用固定在电极上。TH 作为氧化还原探针。在优化条件下，电化学免疫传感器的工作范围从 0.01 到 12 ng/mL，检测限低至 4.86 pg/mL。该免疫传感器的准确性、重现性和稳定性良好。该检测方法用于实际血清样本的评估，结果令人满意。基于纳米多孔金属材料的免疫分析为临床应用提供了一种很有前途的方法，因此代表了一种多功能的检测方法。
