原生动物寄生虫侵袭内阿米巴中瞬时转染和稳定转染
Transient and stable transfection in the protozoan parasite Entamoeba invadens.
作者信息
Ehrenkaufer Gretchen M, Singh Upinder
机构信息
Division of Infectious Diseases, Department of Internal Medicine, Stanford University, Stanford, CA 94305-5107, USA.
出版信息
Mol Biochem Parasitol. 2012 Jul;184(1):59-62. doi: 10.1016/j.molbiopara.2012.04.007. Epub 2012 Apr 24.
Abstract
Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The reptile parasite Entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. However, functional studies using E. invadens have been limited by the lack of genetic tools in this species. Here, we report a new method for both transient and stable transfection of E. invadens. These new tools will greatly enhance research into Entamoeba development.
摘要
溶组织内阿米巴是一种重要的人类病原体，也是全球范围内的一个主要健康问题。除了阶段转换外，寄生虫生物学的许多方面都可以进行研究，而溶组织内阿米巴无法充分再现阶段转换过程。爬行动物寄生虫侵袭内阿米巴是研究阶段转换的重要模型系统，因为它可以在体外高效诱导发生包囊化和脱囊化。然而，由于该物种缺乏遗传工具，使用侵袭内阿米巴进行的功能研究受到了限制。在此，我们报告了一种用于侵袭内阿米巴瞬时转染和稳定转染的新方法。这些新工具将极大地促进对阿米巴发育的研究。
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