Department of Chemistry, Indian Institute of Technology, Kharagpur, Kharagpur 721302, India.
Int J Biol Macromol. 2012 Jul-Aug;51(1-2):1-6. doi: 10.1016/j.ijbiomac.2012.04.024. Epub 2012 May 4.
Copper is known to exert diverse effects on the self-association of proteins and has been found in amyloid deposits that are involved in neurodegenerative disease processes. The effects of the metal ion on the protein during fibrillation were investigated by fluorescence, circular dichroism spectroscopy and fluorescence microscopy. We report for the first time, the complete reduction of Cu(II)→Cu(I) in vitro during fibrillation of hen egg white lysozyme at pH 7. This was confirmed by the lack of any signal for Cu(II) in electron paramagnetic resonance spectroscopy and quantification of Cu(I) was achieved by a bathocuproine disulfonate based assay.
铜被认为对蛋白质的自组装有多种影响，并在参与神经退行性疾病过程的淀粉样沉积物中被发现。通过荧光、圆二色性光谱和荧光显微镜研究了金属离子在纤维形成过程中对蛋白质的影响。我们首次报道了在 pH 7 下鸡卵清溶菌酶纤维形成过程中 Cu(II)→Cu(I)的完全体外还原。这一点通过电子顺磁共振光谱中没有任何 Cu(II)信号得到证实，并通过基于 bathocuproine disulfonate 的测定法对 Cu(I)进行了定量。
