Departamento de Ecología, LINC-Global, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 6513677, Chile.
Comp Biochem Physiol A Mol Integr Physiol. 2012 Aug;162(4):391-6. doi: 10.1016/j.cbpa.2012.04.017. Epub 2012 Apr 27.
The relationship between thermal tolerance and environmental conditions has been extensively studied in Drosophila. However, comparisons of thermal tolerance of laboratory-bred flies derived from distinct geographic locations have produced puzzling results. We studied the differential expression of heat shock protein (HSP) after heat (34 °C) and cold (-4 °C) temperature treatments in two species of Drosophila flies, with distinct biogeographic origins (tropical = D. melanogaster and Andean = D. gaucha), previously exposed to sublethal acclimation temperatures (10, 20 and 30 °C). Also we evaluated the relationship between thermal acclimation and survival value as a proxy of fitness. We found a positive relationship between thermotolerance and the patterns of hsp90 transcript expression in both species. Nevertheless, in the cases in which hsp90 mRNA expression does not match thermotolerance induction, the biogeographic origin of the species could explain such mismatches. Survival at upper and lower experimental temperatures were also related with species origin.
热耐受与环境条件之间的关系在果蝇中已经得到了广泛的研究。然而，对来自不同地理来源的实验室培育的果蝇的热耐受的比较产生了令人困惑的结果。我们研究了在两种果蝇（具有不同生物地理起源的热带果蝇=D. melanogaster 和安第斯果蝇=D. gaucha）中，热（34°C）和冷（-4°C）温度处理后热休克蛋白（HSP）的差异表达，这些果蝇先前已经暴露在亚致死的驯化温度（10、20 和 30°C）下。此外，我们还评估了热驯化与作为适合度替代物的生存值之间的关系。我们发现，在这两个物种中，热耐受与 hsp90 转录物表达模式之间存在正相关关系。然而，在 hsp90 mRNA 表达与热耐受诱导不匹配的情况下，物种的生物地理起源可以解释这种不匹配。在较高和较低实验温度下的生存也与物种起源有关。
