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从开花植物和脊椎动物中对水通道蛋白进化的新认识:同源鉴定和功能转移是可能的。

New insight into the evolution of aquaporins from flowering plants and vertebrates: orthologous identification and functional transfer is possible.

出版信息

Gene. 2012 Jul 15;503(1):165-76. doi: 10.1016/j.gene.2012.04.021. Epub 2012 Apr 24.

Abstract

Aquaporins (AQPs) represent a family of channel proteins that transport water and/or small solutes across cell membranes in the three domains of life. In all previous phylogenetic analysis of aquaporin, trees constructed using proteins with very low amino acid identity (<15%) were incongruent with rRNA data. In this work, restricting the evolutionary study of aquaporins to proteins with high amino acid identity (>25%), we showed congruence between AQPs and organismal trees. On the basis of this analysis, we defined 19 orthologous gene clusters in flowering plant species (3 PIP-like, 7 TIP-like, 6 NIP-like and 3 SIP-like). We described specific conserved motifs for each subfamily and each cluster, which were used to develop a method for automatic classification. Analysis of amino acid identity between orthologous monocotyledon and dicotyledon AQPs from each cluster, suggested that PIPs are under high evolutionary constraint. The phylogenetic analysis allowed us the assignment of orthologous aquaporins for very distant animal lineages (tetrapods-fishes). We also demonstrated that the location of all vertebrate AQPs in the ortholog clusters could be predicted by comparing their amino acid identity with human AQPs. We defined four AQP subfamilies in animals: AQP1-like, AQP8-like, AQP3-like and AQP11-like. Phylogenetic analysis showed that the four animal AQPs subfamilies are related with PIP-like, TIP-like, NIP-like and SIP-like subfamilies, respectively. Thus, this analysis would allow the prediction of individual AQPs function on the basis of orthologous genes from Arabidopsis thaliana and Homo sapiens.

摘要

水通道蛋白(AQP)是一类跨细胞膜转运水和/或小分子溶质的通道蛋白,存在于生命的三个域中。在之前所有的水通道蛋白系统发育分析中,使用氨基酸同一性<15%的蛋白构建的系统树与 rRNA 数据不一致。在这项工作中,我们将水通道蛋白的进化研究限制在氨基酸同一性>25%的蛋白上,结果显示 AQP 与生物体树之间存在一致性。在此基础上,我们在开花植物物种中定义了 19 个同源基因簇(3 个 PIP 样、7 个 TIP 样、6 个 NIP 样和 3 个 SIP 样)。我们描述了每个亚家族和每个簇的特定保守基序,这些基序被用于开发一种自动分类方法。对每个簇中同源单子叶植物和双子叶植物 AQP 的氨基酸同一性进行分析,表明 PIP 受到高度进化限制。系统发育分析使我们能够为非常遥远的动物谱系(四足动物-鱼类)分配同源水通道蛋白。我们还证明,通过比较其与人类 AQP 的氨基酸同一性,可以预测所有脊椎动物 AQP 在同源簇中的位置。我们在动物中定义了四个 AQP 亚家族:AQP1 样、AQP8 样、AQP3 样和 AQP11 样。系统发育分析表明,这四个动物 AQP 亚家族分别与 PIP 样、TIP 样、NIP 样和 SIP 样亚家族相关。因此,这种分析可以根据拟南芥和人类的同源基因来预测单个 AQP 的功能。

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