Department of Physiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
Acta Pharmacol Sin. 2012 Jun;33(6):717-27. doi: 10.1038/aps.2012.32. Epub 2012 May 7.
To determine whether electrical stimulation of caudal medial prefrontal cortex (mPFC) as conditioned stimulus (CS) paired with airpuff unconditioned stimulus (US) was sufficient for establishing eyeblink conditioning in guinea pigs, and whether it was dependent on cerebellar interpositus nucleus.
Thirty adult guinea pigs were divided into 3 conditioned groups, and trained on the delay eyeblink conditioning, short-trace eyeblink conditioning, and long-trace eyeblink conditioning paradigms, respectively, in which electrical stimulation of the right caudal mPFC was used as CS and paired with corneal airpuff US. A pseudo conditioned group of another 10 adult guinea pigs was given unpaired caudal mPFC electrical stimulation and the US. Muscimol (1 μg in 1 μL saline) and saline (1 μL) were infused into the cerebellar interpositus nucleus of the animals through the infusion cannula on d 11 and 12, respectively.
The 3 eyeblink conditioning paradigms have been successfully established in guinea pigs. The animals acquired the delay and short-trace conditioned responses more rapidly than long-trace conditioned responses. Muscimol infusion into the cerebellar interpositus nucleus markedly impaired the expression of the 3 eyeblink conditioned responses.
Electrical stimulation of caudal mPFC is effective CS for establishing eyeblink conditioning in guinea pigs, and it is dependent on the cerebellar interpositus nucleus.
确定尾部内侧前额叶皮质（mPFC）电刺激作为条件刺激（CS）与空气喷溅非条件刺激（US）配对是否足以在豚鼠中建立眨眼条件反射，以及它是否依赖于小脑间位核。
将 30 只成年豚鼠分为 3 个条件组，分别在延迟眨眼条件反射、短迹眨眼条件反射和长迹眨眼条件反射范式中进行训练，其中右侧尾部 mPFC 的电刺激用作 CS，并与角膜空气喷溅 US 配对。另一个 10 只成年豚鼠的假性条件组接受非配对的尾部 mPFC 电刺激和 US。在第 11 天和第 12 天，通过输注套管将 1 μg 毒蕈碱（1 μL 生理盐水）和生理盐水（1 μL）分别输注到动物的小脑间位核中。
在豚鼠中成功建立了 3 种眨眼条件反射范式。动物获得延迟和短迹条件反射的速度快于长迹条件反射。小脑间位核内注射毒蕈碱明显损害了 3 种眨眼条件反射的表达。
尾部 mPFC 的电刺激是在豚鼠中建立眨眼条件反射的有效 CS，它依赖于小脑间位核。
