内源性维生素E在肾脏缺血预处理过程中的作用：雄性和雌性大鼠之间的差异
Role of endogenous vitamin E in renal ischemic preconditioning process: differences between male and female rats.
作者信息
Aryamanesh Simin, Ebrahimi Seyyed Meisam, Abotaleb Nahid, Nobakht Maliheh, Rahimi-Moghaddam Parvaneh
机构信息
Tehran Forensic Medicine, Tehran, Iran.
Abhar School of Nursing, Zanjan University of Medical Sciences, Zanjan, Iran.
出版信息
Iran Biomed J. 2012;16(1):44-51. doi: 10.6091/ibj.983.2012.
BACKGROUND
Antioxidants such as α-tocopherol (vitamin E) and β-carotene (vitamin A) play an important role in protective effect of repeated brief periods of ischemia, namely ischemic preconditioning (IPC). Values of these antioxidants were investigated and compared after induction of ischemia reperfusion (IR) and kidney IPC in both male and female rats.
METHODS
Forty eight Wistar rats were divided randomly into six groups of 8: groups A and B (male and female controls, respectively), group C (male IR or IR cases), group D (female IR cases) and groups E and F (male and female IPC cases, respectively). In groups C and D, ischemia was induced by clamping of left renal arteries for 45 min. In groups E and F, rats underwent four cycles of 4 min of arterial clamping and 11 min of de-clamping before final 45 min ischemia induction. Afterward, serum was collected to assess the blood urea nitrogen, creatinine and vitamins E and A values. Renal tissues were obtained for histological assessments.
RESULTS
α-tocopherol levels in male and female rats showed a significant increase in IPC compared with IR group (P<0.01) and also in female IPC compared with male IPC group. β-carotene levels had no significant variations. Histological evaluation showed that IR-induced renal injuries were less in female rats. Also, protective effects of IPC were more in female rats (P<0.01).
CONCLUSIONS
Renal IPC reduced damages in both male and female rats, but tissue injuries in females were decreased much more along with the increase of endogenous vitamin E.
背景
抗氧化剂如α-生育酚（维生素E）和β-胡萝卜素（维生素A）在反复短暂缺血的保护作用即缺血预处理（IPC）中发挥重要作用。在雄性和雌性大鼠中诱导缺血再灌注（IR）和肾脏IPC后，对这些抗氧化剂的值进行了研究和比较。
方法
48只Wistar大鼠随机分为6组，每组8只：A组和B组（分别为雄性和雌性对照组），C组（雄性IR组或IR病例组），D组（雌性IR病例组）以及E组和F组（分别为雄性和雌性IPC病例组）。在C组和D组中，通过夹闭左肾动脉45分钟诱导缺血。在E组和F组中，大鼠在最终诱导45分钟缺血之前经历4个周期的4分钟动脉夹闭和11分钟松开。之后，收集血清以评估血尿素氮、肌酐以及维生素E和A的值。获取肾组织进行组织学评估。
结果
与IR组相比，雄性和雌性大鼠的α-生育酚水平在IPC组中显著升高（P<0.01），并且与雄性IPC组相比，雌性IPC组中α-生育酚水平也显著升高。β-胡萝卜素水平无显著变化。组织学评估显示，IR诱导的肾损伤在雌性大鼠中较少。此外，可以看出IPC在雌性大鼠中的保护作用更强（P<0.01）。
结论
肾脏IPC减少了雄性和雌性大鼠的损伤，但随着内源性维生素E的增加，雌性大鼠的组织损伤减少得更多。
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