School of Resources and Environmental Sciences, University of Jinan, Jinan, Shandong, 250022, People's Republic of China.
Bioprocess Biosyst Eng. 2012 Nov;35(9):1489-96. doi: 10.1007/s00449-012-0738-9. Epub 2012 May 5.
Aerobic granular sludge was successfully cultivated with the effluent of internal circulation (IC) reactor in a pilot-scale sequencing batch reactor (SBR) using activated sludge as seeding sludge. N removal was investigated in the start-up of aerobic granulation process. Initially, the phenomenon of partial nitrification was observed and nitrite accumulation rates (NO(2) (-)-N/NO (x) (-) -N) were between 84.6 and 99.1 %. It was potentially caused by ammonium oxidizing bacteria (AOB) in the seeding activated sludge, high external environmental temperature (~32 °C) and free ammonia (FA) concentration. After 50 days' running, the aerobic granules-based bioreactor demonstrated perfect performance in simultaneous removal of organic matter and ammonia nitrogen, and average removal efficiencies were maintained above 93 and 96 %, respectively. The maximum nitrogen removal efficiency of 83.1 % was achieved after the formation of aerobic granules. The average diameter of mature aerobic granular sludge mostly ranged from 0.5 to 1.0 mm. Furthermore, one typical cyclic test indicated that pH and DO profiles could be used as effective parameters for biological reactions occurring in the aerobic/anoxic process. The obtained results could provide further information on the cultivation of aerobic granular sludge with practical wastewater, especially with regard to nitrogen-rich industrial wastewater.
采用活性污泥作为接种污泥，在中试规模序批式反应器（SBR）中利用内循环（IC）反应器的出水成功培养出好氧颗粒污泥。在好氧颗粒化过程的启动阶段研究了脱氮效果。最初，观察到部分硝化现象，亚硝酸盐积累率（NO2--N/NOx--N）在 84.6%至 99.1%之间。这可能是由接种活性污泥中的氨氧化细菌（AOB）、较高的外部环境温度（~32°C）和游离氨（FA）浓度引起的。经过 50 天的运行，基于好氧颗粒的生物反应器在同时去除有机物和氨氮方面表现出优异的性能，平均去除率分别保持在 93%和 96%以上。好氧颗粒形成后，最大脱氮效率达到 83.1%。成熟的好氧颗粒污泥的平均直径大多在 0.5 至 1.0 毫米之间。此外，一项典型的循环测试表明，pH 和 DO 曲线可以作为好氧/缺氧过程中生物反应的有效参数。这些结果可以为使用实际废水（特别是富氮工业废水）培养好氧颗粒污泥提供进一步的信息。
