Chengdu Institute of Biology, Chinese Academy of Sciences, China.
Curr Microbiol. 2012 Aug;65(2):128-32. doi: 10.1007/s00284-012-0134-3. Epub 2012 May 6.
Genome shuffling was applied to increase ABE production of the strict anaerobe C. acetobutylicum CICC 8012. By using physical and chemical mutagenesis, strains with superior streptomycin sulfate, 2-deoxy-D-glucose and butanol tolerance levels were isolated. These strains were used for genome shuffling. The best performing strain F2-GA was screened after two rounds of genome shuffling. With 55 g glucose/l as carbon source, F2-GA produced 22.21 g ABE/l in 72 h and ABE yield reached 0.42 g/g which was about 34.53 % improvement compared to the wild type. Fermentation parameters and gene expression of several key enzymes in ABE metabolic pathways were varied significantly between F2-GA and the wild type. These results demonstrated the potential use of genome shuffling to microbial breeding which were difficult to deal with traditional methods.
利用基因组改组技术提高严格厌氧菌丙酮丁醇梭菌 CICC8012 的 ABE 生产。通过物理和化学诱变，筛选得到链霉素硫酸盐、2-脱氧-D-葡萄糖和丁醇耐受性更高的菌株。这些菌株用于基因组改组。经过两轮基因组改组后，筛选出性能最佳的菌株 F2-GA。以 55 g/L 葡萄糖为碳源，F2-GA 在 72 h 内生产 22.21 g/L ABE，ABE 得率达到 0.42 g/g，与野生型相比提高了约 34.53%。F2-GA 与野生型之间 ABE 代谢途径中几种关键酶的发酵参数和基因表达有显著差异。这些结果表明，基因组改组技术在微生物育种中的应用具有潜力，而传统方法很难处理这些微生物。
