Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305-701, Republic of Korea.
J Microbiol. 2012 Dec;50(6):1063-6. doi: 10.1007/s12275-012-2373-1. Epub 2012 Dec 30.
Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. a cetobutylicum medium 1; CAM1). The maximum OD(600) value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19.7, which is 1.8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0.83 g/L/h, which is 1.5 times higher than that (0.55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.
丙酮丁醇梭菌是一种具有工业重要性的生物体，能够生产丙酮-丁醇-乙醇（ABE）。本研究的主要目的是研究在丙酮丁醇梭菌从产酸相到溶剂相转变过程中，细胞密度增加对 ABE 生产的影响。通过使用新设计的培养基（命名为丙酮丁醇梭菌培养基 1；CAM1）来增加细胞密度，从而提高了丙酮丁醇梭菌的 ABE 生产力。使用 CAM1 获得的丙酮丁醇梭菌 ATCC 824 菌株的最大 OD（600）值为 19.7，是使用梭菌生长培养基（CGM）获得的 OD（600）值的 1.8 倍。在 ATCC 824 菌株的 CAM1 发酵中获得的总 ABE 生产力为 0.83 g/L/h，是使用 CGM 获得的 ABE 生产力（0.55 g/L/h）的 1.5 倍。然而，CAM1 获得的生产力增加并没有导致最终 ABE 浓度的增加，因为在高酸浓度下发生了相转变。
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