IEEE Trans Image Process. 2012 Aug;21(8):3518-30. doi: 10.1109/TIP.2012.2197007. Epub 2012 May 1.
We propose to directly process 3D + t image sequences with mathematical morphology operators using a new classification of the 3D+t structuring elements. Several methods (filtering, tracking, segmentation) dedicated to the analysis of 3D + t datasets of zebrafish embryogenesis are introduced and validated through a synthetic dataset. Then, we illustrate the application of these methods to the analysis of datasets of zebrafish early development acquired with various microscopy techniques. This processing paradigm produces spatio-temporal coherent results as it benefits from the intrinsic redundancy of the temporal dimension and minimizes the needs for human intervention in semi-automatic algorithms.
我们建议使用新的 3D+t 结构元素分类，直接对 3D+t 图像序列进行数学形态学操作。本文介绍了几种专门用于分析斑马鱼胚胎发生的 3D+t 数据集的方法（滤波、跟踪、分割），并通过合成数据集进行了验证。然后，我们通过各种显微镜技术获取的斑马鱼早期发育数据集说明了这些方法的应用。由于该处理范例利用了时间维度的固有冗余性并最小化了对半自动化算法的人为干预需求，因此它可以产生时空一致的结果。
