Walser-Reinhard L, Schaarschmidt-Kiener D, Forster J L, Matheis F, Spiess B
Equine Department, Section of Ophathalmology, University of Zurich, Switzerland.
Schweiz Arch Tierheilkd. 2012 Apr;154(4):149-52. doi: 10.1024/0036-7281/a000318.
The following report describes the direct detection of Ehrlichia canis by real-time PCR in the conjunctiva of a 1-year-old female Maltese dog. After being imported from Brazil, the dog was presented because of anorexia, dehydration, fever, and palpable mandibular lymph nodes. A few days later, the dog developed bilateral blepharospasm, photophobia and anterior uveitis. Monocytic ehrlichia was diagnosed by a positive PCR result and the detection of IgM and IgG antibodies. Because of the massive uveitis a conjunctival sample was taken with a cytobrush, which also tested positive for Ehrlichia canis DNA by real-time PCR. Only one week after starting treatment with systemic doxycycline and local anti-inflammatory and cyclopalgic therapy the dog recovered from systemic and eye diseases. After therapy the follow-up examination revealed a full remission of clinical and hematological parameters and negative PCR result.
以下报告描述了通过实时聚合酶链反应（PCR）在一只1岁雌性马尔济斯犬的结膜中直接检测犬埃立克体。这只狗从巴西进口后，因厌食、脱水、发热和可触及的下颌淋巴结而就诊。几天后，这只狗出现双侧眼睑痉挛、畏光和前葡萄膜炎。通过PCR结果阳性以及检测到IgM和IgG抗体，诊断为单核细胞埃立克体感染。由于严重的葡萄膜炎，用细胞刷采集了结膜样本，该样本通过实时PCR检测犬埃立克体DNA也呈阳性。在开始全身使用强力霉素以及局部抗炎和睫状肌麻痹治疗仅一周后，这只狗从全身疾病和眼部疾病中康复。治疗后随访检查显示临床和血液学参数完全缓解，PCR结果为阴性。
