Ohazama Atsushi
Department of Craniofacial Development, Dental Institute, King's College London, London, UK.
Methods Mol Biol. 2012;887:1-13. doi: 10.1007/978-1-61779-860-3_1.
Histology is the study of the microscopic anatomy of tissues by examining a thin slice of the tissue under the microscope. Prior to slicing/sectioning, most tissues require some form of solidifying to allow thin sections to be cut. However, since the tooth is the hardest substance in the vertebrate body, it is one of the most difficult tissues to process for histology. This chapter describes the methods used for making histological sections of tooth from different embryonic stages through to adulthood.
组织学是通过在显微镜下检查组织薄片来研究组织的微观解剖结构。在切片/制片之前,大多数组织需要某种形式的固化,以便能够切出薄片。然而,由于牙齿是脊椎动物体内最坚硬的物质,它是最难进行组织学处理的组织之一。本章描述了从不同胚胎阶段到成年期制作牙齿组织切片所使用的方法。