IQS School of Engineering, Universitat Ramon Llull, Via Augusta, 390, 08017 Barcelona, Spain.
Photochem Photobiol Sci. 2012 Jun;11(6):1099-107. doi: 10.1039/c2pp25105a. Epub 2012 May 8.
The photoprocesses involved in hypericin photoinactivation of three different Candida species (C. albicans, C. parapsilosis and C. krusei) have been examined. Production of singlet oxygen from the triplet state and of superoxide from both the triplet state and the semiquinone radical anion are demonstrated. Hydrogen peroxide is formed downstream of these early events. The outcome of the photodynamic treatments is dictated by the intracellular distribution of hypericin, which is different in the three species and affects the ability of hypericin to produce the different reactive oxygen species and trigger cell-death pathways. The results are in line with the previously-observed different susceptibilities of the three Candida species to hypericin photodynamic treatments.
已研究了金丝桃素光灭活三种不同念珠菌（白念珠菌、近平滑念珠菌和克柔念珠菌）过程中的光化学反应。证明了从三重态产生单线态氧和从三重态和半醌自由基阴离子产生超氧阴离子。这些早期事件的下游会形成过氧化氢。光动力治疗的结果取决于金丝桃素在细胞内的分布，这在三种物种中是不同的，影响了金丝桃素产生不同的活性氧物种和触发细胞死亡途径的能力。这些结果与之前观察到的三种念珠菌对金丝桃素光动力处理的不同敏感性一致。
