• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用 135K StemArray 对人类干细胞中的染色体异常进行高分辨率基因组分析。

High-Resolution Genomic Profiling of Chromosomal Abnormalities in Human Stem Cells Using the 135 K StemArray.

机构信息

Ambry Genetics, Departments of Genomic Services and R&D, 15 Argonaut, Aliso Viejo, CA 92656, USA.

出版信息

Stem Cells Int. 2012;2012:431534. doi: 10.1155/2012/431534. Epub 2012 Apr 5.

DOI:10.1155/2012/431534
PMID:22567024
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3337514/
Abstract

Culturing stem cells for an extended period of time can lead to acquired chromosomal aberrations. Determining the copy number variant (CNV) profile of stem cell lines is critical since CNVs can have dramatic effects on gene expression and tumorigenic potential. Here, we describe an improved version of our StemArray, a stem-cell-focused comparative genomic hybridization (aCGH) microarray, which contains 135,000 probes and covers over 270 stem cell and cancer related genes at the exon level. We have dramatically increased the median probe spacing throughout the genome in order to obtain a higher resolution genetic profile of the cell lines. To illustrate the importance of using the StemArray, we describe a karyotypically normal iPSC line in which we detected acquired chromosomal variations that could affect the cellular phenotype of the cells. Identifying adaptive chromosomal aberrations in stem cell lines is essential if they are to be used in regenerative medicine.

摘要

长时间培养干细胞会导致获得性染色体异常。确定干细胞系的拷贝数变异 (CNV) 谱至关重要，因为 CNVs 会对基因表达和致瘤潜能产生巨大影响。在这里，我们描述了我们的 StemArray 的一个改进版本，这是一种专注于干细胞的比较基因组杂交 (aCGH) 微阵列，其中包含 135000 个探针，涵盖了超过 270 个与干细胞和癌症相关的基因的外显子水平。为了获得细胞系更高分辨率的遗传图谱，我们大幅增加了整个基因组中的中位数探针间距。为了说明使用 StemArray 的重要性，我们描述了一条核型正常的 iPSC 系，我们在其中检测到了可能影响细胞表型的获得性染色体变异。如果要将干细胞系用于再生医学，那么识别适应性染色体异常是至关重要的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/2fed2efb9779/SCI2012-431534.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/b0d0ae3ccee5/SCI2012-431534.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/f9a2817f7672/SCI2012-431534.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/599bf025b020/SCI2012-431534.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/2fed2efb9779/SCI2012-431534.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/b0d0ae3ccee5/SCI2012-431534.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/f9a2817f7672/SCI2012-431534.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/599bf025b020/SCI2012-431534.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf6f/3337514/2fed2efb9779/SCI2012-431534.004.jpg

相似文献

1
High-Resolution Genomic Profiling of Chromosomal Abnormalities in Human Stem Cells Using the 135 K StemArray.使用 135K StemArray 对人类干细胞中的染色体异常进行高分辨率基因组分析。
Stem Cells Int. 2012;2012:431534. doi: 10.1155/2012/431534. Epub 2012 Apr 5.
2
High resolution array-CGH characterization of human stem cells using a stem cell focused microarray.使用干细胞专用微阵列对人类干细胞进行高分辨率 array-CGH 分析。
Mol Biotechnol. 2010 Nov;46(3):234-42. doi: 10.1007/s12033-010-9294-1.
3
Array-comparative genomic hybridization characterization of human pluripotent stem cells.人类多能干细胞的阵列比较基因组杂交特征分析
Methods Mol Biol. 2012;873:261-7. doi: 10.1007/978-1-61779-794-1_17.
4
Detection of submicroscopic chromosomal aberrations by array-based comparative genomic hybridization in fetuses with congenital heart disease.应用基于微阵列的比较基因组杂交技术检测先天性心脏病胎儿的亚微观染色体畸变。
Ultrasound Obstet Gynecol. 2014 Apr;43(4):404-12. doi: 10.1002/uog.13236.
5
Gene copy number variations in adaptive evolution: The genomic distribution of gene copy number variations revealed by genetic mapping and their adaptive role in an undomesticated species, white spruce (Picea glauca).适应性进化中的基因拷贝数变异：通过遗传图谱揭示的基因拷贝数变异的基因组分布及其在未驯化物种白云杉（Picea glauca）中的适应性作用。
Mol Ecol. 2017 Nov;26(21):5989-6001. doi: 10.1111/mec.14337. Epub 2017 Sep 15.
6
Effects of Integrating and Non-Integrating Reprogramming Methods on Copy Number Variation and Genomic Stability of Human Induced Pluripotent Stem Cells.整合型与非整合型重编程方法对人诱导多能干细胞拷贝数变异和基因组稳定性的影响。
PLoS One. 2015 Jul 1;10(7):e0131128. doi: 10.1371/journal.pone.0131128. eCollection 2015.
7
Functionally-focused algorithmic analysis of high resolution microarray-CGH genomic landscapes demonstrates comparable genomic copy number aberrations in MSI and MSS sporadic colorectal cancer.针对高分辨率微阵列比较基因组杂交（microarray-CGH）基因组图谱的功能聚焦算法分析表明，微卫星高度不稳定（MSI）和微卫星稳定（MSS）散发性结直肠癌中存在相当的基因组拷贝数畸变。
PLoS One. 2017 Feb 23;12(2):e0171690. doi: 10.1371/journal.pone.0171690. eCollection 2017.
8
Comprehensive characterization of genomic instability in pluripotent stem cells and their derived neuroprogenitor cell lines.多能干细胞及其衍生的神经祖细胞系中基因组不稳定性的全面表征。
Appl Transl Genom. 2012 Aug 29;1:21-24. doi: 10.1016/j.atg.2012.05.001. eCollection 2012 Dec 1.
9
Micro-scale genomic DNA copy number aberrations as another means of mutagenesis in breast cancer.微尺度基因组 DNA 拷贝数异常作为乳腺癌的另一种诱变手段。
PLoS One. 2012;7(12):e51719. doi: 10.1371/journal.pone.0051719. Epub 2012 Dec 17.
10
Large scale copy number variation (CNV) at 14q12 is associated with the presence of genomic abnormalities in neoplasia.14q12区域的大规模拷贝数变异（CNV）与肿瘤形成中的基因组异常存在相关。
BMC Genomics. 2006 Jun 6;7:138. doi: 10.1186/1471-2164-7-138.

本文引用的文献

1
Recurrent copy number variations in human induced pluripotent stem cells.人诱导多能干细胞中的复发性拷贝数变异
Nat Biotechnol. 2011 Jun 7;29(6):488-91. doi: 10.1038/nbt.1890.
2
Karotypic abnormalities in human induced pluripotent stem cells and embryonic stem cells.人类诱导多能干细胞和胚胎干细胞中的核型异常。
Nat Biotechnol. 2011 Apr;29(4):313-4. doi: 10.1038/nbt.1835.
3
Loss at chromosome arm 16q in retinoblastoma: confirmation of the association with diffuse vitreous seeding and refinement of the recurrently deleted region.
视网膜母细胞瘤 16q 染色体臂缺失：与弥漫性玻璃体播散的关联得到确认，并对反复缺失区域进行了精细化研究。
Genes Chromosomes Cancer. 2011 May;50(5):327-37. doi: 10.1002/gcc.20857. Epub 2011 Feb 8.
4
Identification and classification of chromosomal aberrations in human induced pluripotent stem cells.人类诱导多能干细胞中染色体畸变的鉴定和分类。
Cell Stem Cell. 2010 Oct 8;7(4):521-31. doi: 10.1016/j.stem.2010.07.017.
5
Cadherin 13 in cancer.钙黏蛋白 13 与癌症。
Genes Chromosomes Cancer. 2010 Sep;49(9):775-90. doi: 10.1002/gcc.20787.
6
High resolution array-CGH characterization of human stem cells using a stem cell focused microarray.使用干细胞专用微阵列对人类干细胞进行高分辨率 array-CGH 分析。
Mol Biotechnol. 2010 Nov;46(3):234-42. doi: 10.1007/s12033-010-9294-1.
7
Dppa2 knockdown-induced differentiation and repressed proliferation of mouse embryonic stem cells.Dppa2 敲低诱导的小鼠胚胎干细胞分化和增殖抑制。
J Biochem. 2010 Feb;147(2):265-71. doi: 10.1093/jb/mvp161. Epub 2009 Oct 20.
8
The pluripotency-associated gene Dppa4 is dispensable for embryonic stem cell identity and germ cell development but essential for embryogenesis.多能性相关基因Dppa4对于胚胎干细胞特性和生殖细胞发育并非必需，但对胚胎发生至关重要。
Mol Cell Biol. 2009 Jun;29(11):3186-203. doi: 10.1128/MCB.01970-08. Epub 2009 Mar 30.
9
Recurrent chromosomal abnormalities in human embryonic stem cells.人类胚胎干细胞中的复发性染色体异常。
Nat Biotechnol. 2008 Dec;26(12):1361-3. doi: 10.1038/nbt.1510. Epub 2008 Nov 23.
10
Genetic and epigenetic inactivation of T-cadherin in human hepatocellular carcinoma cells.人肝癌细胞中T-钙黏蛋白的遗传和表观遗传失活
Int J Cancer. 2008 Sep 1;123(5):1043-52. doi: 10.1002/ijc.23634.