Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, 1000 Ljubljana, Slovenia.
J Anal Methods Chem. 2012;2012:379724. doi: 10.1155/2012/379724. Epub 2012 Mar 19.
The most commonly used technique for water content determination is Karl-Fischer titration with electrometric detection, requiring specialized equipment. When appropriate equipment is not available, the method can be performed through visual detection of a titration endpoint, which does not enable an analysis of colored samples. Here, we developed a method with spectrophotometric detection of a titration endpoint, appropriate for moisture determination of colored samples. The reaction takes place in a sealed 4 ml cuvette. Detection is performed at 520 nm. Titration endpoint is determined from the graph of absorbance plotted against titration volume. The method has appropriate reproducibility (RSD = 4.3%), accuracy, and linearity (R(2) = 0.997).
最常用于测定水分含量的技术是带有电化学检测的卡尔费休滴定法，需要专门的设备。当没有合适的设备时，可以通过目视检测滴定终点来进行该方法，但这种方法不能分析有色样品。在这里，我们开发了一种带有分光光度检测滴定终点的方法，适用于有色样品的水分测定。反应在一个密封的 4ml 比色皿中进行。检测在 520nm 处进行。通过绘制吸光度与滴定体积的关系图来确定滴定终点。该方法具有适当的重现性（RSD=4.3%）、准确性和线性（R(2)=0.997）。
