Chinese Academy of Inspection and Quarantine, A3, North Gaobeidian Road, Chaoyang District, Beijing 100123, China.
J Anal Methods Chem. 2012;2012:816180. doi: 10.1155/2012/816180. Epub 2012 Mar 11.
Alphalactalbumin (α-La) and betalactoglobulin (β-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. The effects of different factors, including the surfactant concentration, sample volume, electrolyte, and pH were discussed. The optimized conditions for cloud point extraction of alphalactalbumin (α-La) and betalactoglobulin (β-Lg) can be concluded that the best surfactant is 1% (w/v) Triton X-114, 200 μL of sample volume, 150 mmol/L NaCl, and 6% (w/v) sucrose. After cloud point extraction, the capillary electrophoresis is used to check the efficiency of the extraction procedure. The results had been effectively confirmed by the characterization with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
采用浊点萃取法，利用非离子表面活性剂 Triton X-114 成功分离了牛初乳粉中的α-乳白蛋白（α-La）和β-乳球蛋白（β-Lg）。讨论了不同因素，包括表面活性剂浓度、样品体积、电解质和 pH 值对浊点萃取的影响。优化的α-La 和 β-Lg 浊点萃取条件可以得出，最佳表面活性剂为 1%（w/v）Triton X-114，样品体积 200 μL，150 mmol/L NaCl 和 6%（w/v）蔗糖。浊点萃取后，采用毛细管电泳检查萃取过程的效率。结果通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的表征得到了有效证实。
