Rapoport V L, Malkin V M, Savina A V, Safargaleeva E A, Goriuchko V V
Biofizika. 2012 Jan-Feb;57(1):14-20.
Luminescence and excitation spectra of the highly luminescent stacking dimers of adenine and uracil in water solutions are studied. By the luminescence excitation spectra method it is shown that the stacking aggregates of adenine and uracil are formed with participation of rare forms of monomer molecules: N7H tautomers of adenine and the uracil molecules in rare forms of hydratation, for example molecules without H-bonds with water. The study of temperature dependence of luminescence intensity of monomers and stacking dimers of uracil has shown that stacking dimers do not dissociate even at 85 degrees C similarly as described earlier for adenine and adenosine. Stable stacking aggregates of nucleic bases are most likely to be the precursors of RNA molecules in chemical evolution. This hypothesis is supported by new data on their stability.
研究了水溶液中腺嘌呤和尿嘧啶的高发光堆叠二聚体的发光光谱和激发光谱。通过发光激发光谱法表明，腺嘌呤和尿嘧啶的堆叠聚集体是由单体分子的稀有形式参与形成的：腺嘌呤的N7H互变异构体和处于稀有水合形式的尿嘧啶分子，例如不与水形成氢键的分子。对尿嘧啶单体和堆叠二聚体发光强度的温度依赖性研究表明，堆叠二聚体即使在85摄氏度时也不会解离，这与之前对腺嘌呤和腺苷的描述类似。核酸碱基的稳定堆叠聚集体很可能是化学进化中RNA分子的前体。这一假设得到了关于它们稳定性的新数据的支持。
