Haruta Chihiro
Department of Hard Tissue Engineering, Division of Bio-Matrix, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 2012 Mar;79(1):7-14.
A functional endocannabinoid system is present in several mammalian organs and tissues. The endogenous cannabinoid anandamide (AEA) is a bioactive lipid neurotransmitter that is present in a variety of tissues and has multiple functions. Recently, this cannabinoid system was reported to play important roles in osteoblastic and osteoclastic cells. Human periodontal ligament (hPDL) cells modulate alveolar bone remodeling by producing cytokines when stimulated by many factors. The aim of this study was to investigate the AEA level in periodontal regions and the osteogenic effect of AEA on hPDL cells. The levels of AEA in the gingival crevicular fluid (GCF) of periodontitis patients were measured using liquid chromatography/mass spectrometry (LC/MS). Expressions of cannabinoid receptor mRNA were detected by RT-PCR in hPDL cells and expression of the transient receptor potential cation channel subfamily V member 1 (TRPV1) was observed by confocal laser scanning microscope (CLSM). IL-11 production from hPDL cells was measured using an ELISA, with or without AEA in the presence or absence of capsazepine, a selective TRPV1 antagonist AEA secreted into GCF was detected, but there was no correlation between the probing pocket depth (PPD) and AEA level. TRPV1 mRNA was detected in hPDL cells and the TRPV1 expression was observed by CLSM. IL-11 production from hPDL cells was significantly enhanced by AEA stimulation and this IL-11 production was suppressed by capsazepine. Our findings indicate that this endogenous cannabinoid system has a possible role in bone metabolism in periodontitis through TRPV1.
功能性内源性大麻素系统存在于多种哺乳动物器官和组织中。内源性大麻素花生四烯酸乙醇胺（AEA）是一种生物活性脂质神经递质，存在于多种组织中并具有多种功能。最近，据报道该大麻素系统在成骨细胞和破骨细胞中发挥重要作用。人牙周膜（hPDL）细胞在受到多种因素刺激时会通过产生细胞因子来调节牙槽骨重塑。本研究的目的是调查牙周区域中AEA的水平以及AEA对hPDL细胞的成骨作用。使用液相色谱/质谱联用（LC/MS）测定牙周炎患者龈沟液（GCF）中AEA的水平。通过RT-PCR检测hPDL细胞中大麻素受体mRNA的表达，并通过共聚焦激光扫描显微镜（CLSM）观察瞬时受体电位阳离子通道亚家族V成员1（TRPV1）的表达。使用酶联免疫吸附测定（ELISA）测量hPDL细胞产生的IL-11，在有或没有AEA的情况下，同时存在或不存在选择性TRPV1拮抗剂辣椒素。检测到分泌到GCF中的AEA，但探诊深度（PPD）与AEA水平之间没有相关性。在hPDL细胞中检测到TRPV1 mRNA，并通过CLSM观察到TRPV1的表达。AEA刺激显著增强了hPDL细胞产生的IL-11，而这种IL-11的产生被辣椒素抑制。我们的研究结果表明，这种内源性大麻素系统可能通过TRPV1在牙周炎的骨代谢中发挥作用。
