Nakanishi Toyofumi, Ito Minako, Ueda Kazuhito, Wada Shinichi, Fujioka Shigekazu, Tsuji Motomu, Takubo Takayuki
Department of Clinical and Laboratory Medicine, Osaka Medical College, Takatsuki 569-8686, Japan.
Rinsho Byori. 2012 Feb;60(2):125-30.
MALDI-imaging MS (IMS) with MSMS analysis is a new powerful tool for the identification of not only disease-related proteins in formalin-fixed paraffin-embedded (FFPE) tissue sections but also protein/peptides/drugs/medicine in fresh-frozen tissues. IMS is used to reveal the mass profiles and spatial distribution of proteins in tissue sections and/or digested peptides derived from deposited protein in pathologic organs and then MSMS analysis identifies the amino acid sequence of the detected proteins in the tissue section. Moreover, on-tissue digestion combined with the MALDI-IM-TOF-IMS approach allows a proteomics "bottom-up" strategy with clinical samples, especially perioperative isolated tissues and FFPE tissues conserved for a long time in a clinical sample bank. The mass barcode-like image (MBI) on a longitudinal sliced hair by IMS is used in the selected reaction monitoring mode for serially chronological monitoring and traceability every few hours after drug and medicine intake. The advances of quantitative MBI for sliced sections of hair allow a new universal standardized assessment of drugs and medicines throughout the drug history.
带有串联质谱分析的基质辅助激光解吸电离成像质谱（MALDI-IMS）是一种强大的新工具，不仅可用于鉴定福尔马林固定石蜡包埋（FFPE）组织切片中与疾病相关的蛋白质，还可用于鉴定新鲜冷冻组织中的蛋白质/肽/药物/药品。IMS用于揭示组织切片中蛋白质的质量图谱和空间分布，以及病理器官中沉积蛋白质衍生的消化肽，然后串联质谱分析确定组织切片中检测到的蛋白质的氨基酸序列。此外，组织上消化与MALDI-IM-TOF-IMS方法相结合，可对临床样本采用蛋白质组学“自下而上”策略，特别是围手术期分离组织和在临床样本库中长时间保存的FFPE组织。通过IMS在纵向切片毛发上获得的质量条形码样图像（MBI）用于选择反应监测模式，以便在摄入药物和药品后的数小时内进行连续的时间顺序监测和溯源。毛发切片定量MBI的进展使得能够对整个用药史中的药物和药品进行新的通用标准化评估。
