[富血小板血浆注射对大鼠跟腱断裂早期愈合的影响]

[Effect of platelet-rich plasma injection on early healing of Achilles tendon rupture in rats].

作者信息

Xiong Xiaolong, Wu Liang, Xiang Dayong, Ni Guoxin, Zhao Peiran, Yu Bin

机构信息

Department of Trauma and Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou Guangdong, 510515, P.R. China.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 Apr;26(4):466-71.

PMID:22568331

Abstract

OBJECTIVE

Platelet-rich plasma (PRP) can promote wound healing. To observe the effect of PRP injection on the early healing of rat's Achilles tendon rupture so as to provide the experimental basis for clinical practice.

METHODS

Forty-six Sprague Dawley rats were included in this experiment, female or male and weighing 190-240 g. PRP and platelet-poor plasma (PPP) were prepared from the heart arterial blood of 10 rats; other 36 rats were made the models of Achilles tendon rupture, and were randomly divided into 3 groups (control group, PPP group, and PRP group), 12 rats for each group. In PPP and PRP groups, PPP and PRP of 100 microL were injected around the tendons once a week, respectively; in the control group, nothing was injected. The tendon tissue sample was harvested at 1, 2, 3, and 4 weeks after operation for morphology, histology, and immunohistochemistry observations. The content of collagen type I fibers also was measured. Specimens of each group were obtained for biomechanical test at 4 weeks.

RESULTS

All the animals survived till the end of the experiment. Tendon edema gradually decreased and sliding improved with time. The tendon adhesion increased steadily from 1 week to 3 weeks postoperatively, and it was relieved at 4 weeks in 3 groups. There was no significant difference in the grading of tendon adhesion among 3 groups at 1 week and at 4 weeks (P > 0.05), respectively. The inflammatory cell infiltration, angiogenesis, and collagen fibers were more in PRP group than in PPP group and control group at 1 week; with time, inflammatory cell infiltration and angiogenesis gradually decreased. Positive staining of collagen type I fibers was observed at 1-4 weeks postoperatively in 3 groups. The positive density of collagen type I fibers in group PRP was significantly higher than that in control group and PPP group at 1, 2, and 3 weeks (P < 0.05), but no significant difference was found among 3 groups at 4 weeks (P > 0.05). The biomechanical tests showed that there was no significant difference in the maximal gliding excursion among 3 groups at 4 weeks postoperatively (P > 0.05); the elasticity modulus and the ultimate tensile strength of PRP group were significantly higher than those of control group and PPP group at 4 weeks (P < 0.05).

CONCLUSION

PRP injection can improve the healing of Achilles tendon in early repair of rat's Achilles tendon rupture.

摘要

目的

富血小板血浆（PRP）可促进伤口愈合。观察PRP注射对大鼠跟腱断裂早期愈合的影响，为临床实践提供实验依据。

方法

本实验纳入46只体重190 - 240 g的Sprague Dawley大鼠，雌雄不限。从10只大鼠的心脏动脉血中制备PRP和贫血小板血浆（PPP）；其余36只大鼠制作跟腱断裂模型，并随机分为3组（对照组、PPP组和PRP组），每组12只。在PPP组和PRP组中，分别每周一次在肌腱周围注射100 μL的PPP和PRP；对照组不进行注射。在术后1、2、3和4周采集肌腱组织样本进行形态学、组织学和免疫组织化学观察。同时测量I型胶原纤维的含量。在4周时获取每组标本进行生物力学测试。

结果

所有动物均存活至实验结束。肌腱水肿随时间逐渐减轻，滑动情况改善。术后1周~ 3周肌腱粘连稳步增加，3组在4周时粘连均减轻。3组在1周和4周时肌腱粘连分级分别比较，差异均无统计学意义（P > 0.05）。术后1周时，PRP组的炎症细胞浸润、血管生成及胶原纤维均多于PPP组和对照组；随时间推移，炎症细胞浸润和血管生成逐渐减少。3组术后1 - 4周均可见I型胶原纤维阳性染色。PRP组在术后1、2和3周时I型胶原纤维阳性密度显著高于对照组和PPP组（P < 0.05），但4周时3组间差异无统计学意义（P > 0.05）。生物力学测试显示，术后4周时3组间最大滑动偏移差异无统计学意义（P > 0.05）；4周时PRP组的弹性模量和极限抗拉强度显著高于对照组和PPP组（P < 0.05）。