Ahmad I, Redmond L J, Barnstable C J
Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT 06510.
Biochem Biophys Res Commun. 1990 Nov 30;173(1):463-70. doi: 10.1016/s0006-291x(05)81081-2.
Probes against the retinal cGMP-gated cation channel were generated by PCR amplification of cDNA from rat and bovine retina. Southern and Northern analyses showed that the channel is encoded by a single gene that gives rise to a single mRNA species of 3.2 kb. Low levels of cGMP-gated channel RNA were detected in postnatal day 1 (PN 1) retinas and the amount increased to adult levels over the next two weeks of development. Screening of a number of tissues by Northern blot hybridization and by PCR amplification showed the channel to be expressed by heart and kidney as well as retina, but not by cerebellum, cerebral cortex, liver, muscle, olfactory bulb, spleen, testes or thymus.
通过对大鼠和牛视网膜的cDNA进行PCR扩增，制备了针对视网膜cGMP门控阳离子通道的探针。Southern和Northern分析表明，该通道由一个单一基因编码，产生一种3.2 kb的单一mRNA。在出生后第1天（PN 1）的视网膜中检测到低水平的cGMP门控通道RNA，在接下来的两周发育过程中，其含量增加到成年水平。通过Northern印迹杂交和PCR扩增对多个组织进行筛选，结果显示该通道在心脏、肾脏以及视网膜中表达，但在小脑、大脑皮层、肝脏、肌肉、嗅球、脾脏、睾丸或胸腺中不表达。
