Gantz B J, Abramson M, Huang C C
Otolaryngology. 1978 Mar-Apr;86(2):ORL236-48. doi: 10.1177/019459987808600211.
Bone destruction, commonly associated with chronic otitis media, requires collagen degradation. Collagenase, a neutral protease, appears to be an essential component in the process of collagen breakdown. Collagenase was identified within chronically inflamed and normal guinea pig temporal bones using an immunohistochemical technique with fluorescein isothyocyanate and peroxidase-antiperoxidase labels. Localization of the enzyme identifies sites of matrix resorption. Collagenase was found in osteoclasts, osteocytes, mononuclear inflammatory cells, and at resorbing margins. Inflammation increased the intracellular collagenase content of inflammatory bone osteocytes when compared to normal osteocytes using a microspectrofluorometer. It appears that the inflammatory process directly influences bone destruction through the action of mononuclear inflammatory cells and indirectly by stimulating bone cells to increase their proteolytic enzyme production.
骨破坏通常与慢性中耳炎相关，需要胶原蛋白降解。胶原酶作为一种中性蛋白酶，似乎是胶原蛋白分解过程中的关键成分。利用异硫氰酸荧光素和过氧化物酶 - 抗过氧化物酶标记的免疫组织化学技术，在慢性炎症和正常豚鼠颞骨中鉴定出了胶原酶。该酶的定位确定了基质吸收的部位。在破骨细胞、骨细胞、单核炎性细胞以及吸收边缘均发现了胶原酶。与正常骨细胞相比，使用显微分光荧光计发现炎症会增加炎性骨骨细胞的细胞内胶原酶含量。看来炎症过程通过单核炎性细胞的作用直接影响骨破坏，并且通过刺激骨细胞增加其蛋白水解酶的产生间接影响骨破坏。
