比较牛初乳抗体和各免疫球蛋白类对大肠杆菌 O157:H7 肠毒素 2、鞭毛和体细胞的疗效在小鼠体内。

Comparison of efficacies of bovine immune colostral antibody and each immunoglobulin class against verotoxin 2, flagellum and somatic cells of Escherichia coli O157:H7 in mice.

机构信息

Laboratory of Immunology, School of Life and Environmental Science, Azabu University, Kanagawa, Japan.

出版信息

J Microbiol Immunol Infect. 2013 Apr;46(2):73-9. doi: 10.1016/j.jmii.2012.01.002. Epub 2012 May 7.

DOI:10.1016/j.jmii.2012.01.002

PMID:22572001

Abstract

PURPOSE

The efficacy of bovine immune colostral (colostral) antibodies against verotoxin (VT) 2, flagellum and somatic cells of Escherichia coli (E. coli) O157:H7 in mice was determined.

METHODS

Three major immunoglobulin (Ig) classes were isolated from the colostral antibody against VT2 by affinity chromatography and were used for estimation. Mice inoculated with VT2 were administered each Ig class from the colostral antibody, colostral antibody (colostral whey containing antibody) or serum antibody against VT2 at 1 hour after VT2 inoculation.

RESULTS

All control mice (20/20) died after administration of sterilized saline instead of the colostral antibody. The survival rate was 93.3% (14/15) after administration of S-IgA or IgM antibody, or colostral antibody. Survival rates for IgG antibody and serum antibody administration were 80% (12/15) and 60% (9/15), respectively. Serum concentrations of VT2, which was absorbed from the small intestine in mice after administration of VT2 and colostral antibody, were measured by fluorescence enzyme immunoassay (FEIA). Serum concentrations of VT2 after administration of colostral antibody were lower than those after administration of sterilized saline. Mice inoculated with VT2-producing E. coli 157:H7 were administered anti-flagellum or anti-somatic colostral antibodies. Survival rates for E. coli O157:H7-infected mice administered the anti-flagellum and anti-somatic colostral antibodies were 52.4% (11/21) and 22.2% (4/18), respectively. Furthermore, survival rates increased to 89.5% (17/19) with combined administration of anti-flagellum and anti-VT2 colostral antibodies.

CONCLUSION

These results suggest that colostral antibodies against VT2, flagellum and somatic cells are effective against E. coli O157:H7 infection.

摘要

目的

研究牛初乳（colostrum）抗体对产志贺样毒素（verotoxin）2（VT2）、鞭毛和大肠杆菌（Escherichia coli）O157:H7 体细胞的疗效。

方法

通过亲和层析从 VT2 牛初乳抗体中分离出 3 种主要免疫球蛋白（Ig）类，并进行估计。在接种 VT2 后 1 小时，用 VT2 牛初乳抗体、牛初乳抗体（含抗体的牛初乳乳清）或血清抗体中的每种 Ig 类给接种 VT2 的小鼠进行给药。

结果

所有对照小鼠（20/20）在给予无菌生理盐水而不是牛初乳抗体后均死亡。用 S-IgA 或 IgM 抗体或牛初乳抗体给药后，存活率为 93.3%（14/15）。用 IgG 抗体和血清抗体给药的存活率分别为 80%（12/15）和 60%（9/15）。通过荧光酶免疫分析（FEIA）测量 VT2 在小鼠小肠吸收后的血清浓度。给予牛初乳抗体后的 VT2 血清浓度低于给予无菌生理盐水后的浓度。用产 VT2 的大肠杆菌 157:H7 接种小鼠后，给予抗鞭毛和抗体细胞牛初乳抗体。用抗鞭毛和抗体细胞牛初乳抗体治疗感染大肠杆菌 O157:H7 的小鼠的存活率分别为 52.4%（11/21）和 22.2%（4/18）。此外，联合给予抗鞭毛和抗 VT2 牛初乳抗体后，存活率增加到 89.5%（17/19）。