School of Life Science and Biotechnology, Kyungpook National University, Sankyukdong, Bookgu, Daegu 702-701, Republic of Korea.
J Microbiol Biotechnol. 2012 Jun;22(6):866-71. doi: 10.4014/jmb.1111.11010.
A novel cholesterol-based cationic lipid containing a tri-2- hydroxyethylamine head group and ether linker (Chol- THEA) was synthesized and examined as a potent gene delivery vehicle. In the preparation of cationic liposome, the addition of DOPE as helper lipid significantly increased the transfection efficiency. To find the optimum transfection efficiency, we screened various weight ratios of DOPE and liposome/DNA (N/P). The best transfection efficiency was found at the Chol-THEA:DOPE weight ratio of 1:1 and N/P weight ratio of 10~15. Most of the plasmid DNA was retarded by this liposome at the optimum N/P weight ratio of 10. The transfection efficiency of Chol-THEA liposome was compared with DOTAP, Lipofectamine, and DMRIE-C using the luciferase assay and GFP expression. Chol-THEA liposome with low toxicity had better or similar potency of gene delivery compared with commercial liposomes in COS-7, Huh-7, and MCF-7 cells. Therefore, Chol-THEA could be a useful non-viral vector for gene delivery.
一种新型胆固醇阳离子脂质体，含有三羟乙基胺头基和醚键连接基团（Chol- THEA），被合成并作为一种有效的基因传递载体进行了研究。在阳离子脂质体的制备中，添加 DOPE 作为辅助脂质可显著提高转染效率。为了找到最佳的转染效率，我们筛选了各种 DOPE 和脂质体/DNA（N/P）的重量比。在 Chol- THEA：DOPE 重量比为 1：1 和 N/P 重量比为 10-15 时，发现了最佳的转染效率。在最佳的 N/P 重量比为 10 时，大多数质粒 DNA 被这种脂质体阻滞。用荧光素酶测定法和 GFP 表达比较了 Chol- THEA 脂质体与 DOTAP、Lipofectamine 和 DMRIE-C 的转染效率。Chol- THEA 脂质体毒性低，与商业脂质体在 COS-7、Huh-7 和 MCF-7 细胞中的基因传递效果相当或更好。因此，Chol- THEA 可能是一种有用的非病毒基因传递载体。
