利用光不稳定的张力炔烃构建物进行叠氮标记生物分子的捕获和释放的策略。
Strategy for catch and release of azide-tagged biomolecules utilizing a photolabile strained alkyne construct.
机构信息
Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 18, 72076 Tübingen, Germany.
出版信息
Org Biomol Chem. 2012 Jun 21;10(23):4496-9. doi: 10.1039/c2ob25440a. Epub 2012 May 9.
PMID:22573264
Abstract
A photolabile o-nitrobenzyl linker-cyclooctyne conjugate was prepared, immobilized on poly(methacrylate) beads and utilized as a trap for azide-functionalized compounds. These could be released by 365 nm UV light irradiation in high yield and purity. The "reagent-free" and time economic catch and release protocol was deemed useful for chemical proteomics applications.
摘要
一种光不稳定的邻硝基苄基连接体-环辛炔缀合物被制备出来，固定在聚（甲基丙烯酸酯）珠上，并用作叠氮基功能化合物的捕获物。这些化合物可以通过 365nm 的紫外光照射以高产率和高纯度释放。这种“无试剂”和时间经济的捕获和释放方案被认为对化学蛋白质组学应用有用。
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