Jackson C L, Hartwell L H
Department of Genetics, University of Washington, Seattle 98195.
Cell. 1990 Nov 30;63(5):1039-51. doi: 10.1016/0092-8674(90)90507-b.
We demonstrate that during the courtship stage of conjugation, S. cerevisiae a cells choose the alpha cell producing the highest level of pheromone from among potential mating partners. From this result and that for alpha cells we conclude that both a and alpha cells act as signaling cells during courtship, that both cell types respond by discriminating different levels of signal, and that the signals are the mating pheromones. Responding cells that are supersensitive to signal fail to discriminate pheromone-producing from nonproducing cells to an extent that depends on their degree of supersensitivity. We propose that partner selection in S. cerevisiae results from polarized morphogenesis of a responding cell in the direction of highest pheromone concentration and that cells defective in discriminating this gradient execute a default pathway in which an adjacent cell is selected at random.
我们证明，在接合的求偶阶段，酿酒酵母a细胞会从潜在的交配伙伴中选择产生最高水平信息素的α细胞。基于此结果以及α细胞的相关结果，我们得出结论：在求偶过程中，a细胞和α细胞均作为信号细胞，两种细胞类型都通过区分不同水平的信号做出反应，且这些信号就是交配信息素。对信号超敏感的反应细胞无法在一定程度上区分产生信息素的细胞和不产生信息素的细胞，这种程度取决于它们的超敏感程度。我们提出，酿酒酵母中的伴侣选择是由反应细胞在最高信息素浓度方向上的极化形态发生导致的，而在区分这种梯度方面存在缺陷的细胞会执行默认途径，即随机选择相邻细胞。
