Wang Taylor K, Keller Abigail, Kunjo Omar, Dunham Maitreya J
Department of Genome Sciences, University of Washington, Seattle, WA, USA.
bioRxiv. 2025 Jun 4:2025.06.02.657496. doi: 10.1101/2025.06.02.657496.
The field of budding yeast research has long been empowered by the vast array of genetic tools and community resources available. Rescue by mating is one useful tool that entails mating of meiotic spores directly post-germination. This minimal cell division mating process facilitates mating based screens studying the effects of otherwise haploid lethal gene deletions. In this study, we describe the successful application of rescue by mating across two different species: and . This novel inter-species tool enables studies on the evolution of essential genes within the broader genus.
长期以来，丰富的遗传工具和社区资源推动了芽殖酵母研究领域的发展。通过交配进行拯救是一种有用的工具，它需要在减数分裂孢子萌发后直接进行交配。这种最少细胞分裂的交配过程有助于基于交配的筛选，研究否则为单倍体致死基因缺失的影响。在本研究中，我们描述了通过交配在两个不同物种： 和 之间的成功应用。这种新型的种间工具能够在更广泛的 属内对必需基因的进化进行研究。
