Zhang Chun-Xia, Sun Lei, Xiu Zhong-Biao
The Peoples Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, Fujian, China.
Zhongguo Gu Shang. 2012 Feb;25(2):138-42.
To observe the influence of Pilose antler polypeptide on the glycosaminoglycan and type II collagen in the articular cartilage in experimental knee osteoarthritis.
Totally 64 New Zealand white rabbits of 6 months old were randomly divided into 2 groups:normal group (n = 8) and model group (n = 56). Model group was surgically induced into osteoarthritis model by method of Hulth. After successful modeling, the rabbits of model group were further divided into 2 groups: Pilose antler polypeptide-treatment group and control group, 24 rabbits in each group. Pilose antler polypeptide-treatment group received 0.5 ml intra-articular injection of Pilose antler polypeptide dilution liquid once in per 2 days for 30 days, while control group received 0.5 ml intra-articular injection of physiological saline. On days 7, 15 and 30 after intervention, articular cartilage samples were collected respectively. The content of glycosaminoglycan in articular cartilage was observed by toluidine blue staining and the expression of type II collagen in cartilage matrix was detected by immunohistochemical staining.
Along with the prolonging of time, the content of glycosaminoglycan and type II collagen in cartilage matrix of the Pilose antler polypeptide-treatment group and control group decreased gradually. On days 7, 15 and 30 after intervention, integrated optical density of the type II collagen positive area in cartilage matrix of the Pilose antler polypeptide-treatment group were (312.06 +/- 14.12), (273.31 +/- 12.42) and (248.34 +/- 10.41), which had statistically significant differences. Integrated optical density of the type II collagen positive area in cartilage matrix of the control group were (253.47 +/- 15.53), (215.67 +/- 9.72) and (160.01 +/- 13.23), which had statistically significant differences. At the same period, integrated optical density of the type II collagen positive area in cartilage matrix of the Pilose antler polypeptide-treatment group was higher than that of control group, which had statistically significant difference.
Pilose antler polypeptide can inhibit reduction of the glycosaminoglycan and type II collagen in cartilage matrix and delay the degeneration of articular cartilage.
观察鹿茸多肽对实验性膝骨关节炎关节软骨中糖胺聚糖及Ⅱ型胶原蛋白的影响。
选取64只6月龄新西兰白兔，随机分为2组：正常组（n = 8）和模型组（n = 56）。模型组采用Hulth法手术诱导建立骨关节炎模型。造模成功后，将模型组家兔进一步分为2组：鹿茸多肽治疗组和对照组，每组24只。鹿茸多肽治疗组每2天关节腔内注射0.5 ml鹿茸多肽稀释液，共30天，对照组关节腔内注射0.5 ml生理盐水。干预后第7、15、30天分别采集关节软骨样本。采用甲苯胺蓝染色观察关节软骨中糖胺聚糖含量，免疫组织化学染色检测软骨基质中Ⅱ型胶原蛋白表达。
随着时间延长，鹿茸多肽治疗组和对照组软骨基质中糖胺聚糖及Ⅱ型胶原蛋白含量逐渐降低。干预后第7、15、30天，鹿茸多肽治疗组软骨基质中Ⅱ型胶原蛋白阳性区域的积分光密度分别为（312.06±14.12）、（273.31±12.42）和（248.34±10.41），差异有统计学意义。对照组软骨基质中Ⅱ型胶原蛋白阳性区域的积分光密度分别为（253.47±15.53）、（215.67±9.72）和（160.01±13.23），差异有统计学意义。同期，鹿茸多肽治疗组软骨基质中Ⅱ型胶原蛋白阳性区域的积分光密度高于对照组，差异有统计学意义。
鹿茸多肽可抑制软骨基质中糖胺聚糖及Ⅱ型胶原蛋白含量的降低，延缓关节软骨退变。
