PrimeraDx, Mansfield, MA, USA.
Biotechniques. 2012 May;52(5):316-24. doi: 10.2144/0000113852.
Quantitative PCR (qPCR) using real-time detection of amplification is limited to a small number of targets within a single reaction. The ICEPlex system, using our scalable target analysis routine (STAR) technology, was developed to provide an automated, high multiplexing PCR solution. ICEPlex combines PCR thermal cycling with dynamic, sequential amplicon separation by capillary electrophoresis and two-color quantitative detection in a single integrated system. In contrast to probe-based qPCR, ICEPlex directly measures amplicon accumulation through incorporation of labeled primers. Three orders of magnitude of optical detection range and at least 7 logs of detectable target concentration range are demonstrated. The system can separate more than 50 amplicons per color channel, ranging from 100 to 500 bases, providing broad multiplexing capabilities for a wide spectrum of nucleic acid amplification applications. ICEPlex can be used for analysis of viral DNA or RNA targets, detection of genetic variants, and for reverse-transcriptase PCR gene expression panels.
实时荧光定量 PCR（qPCR）在单个反应中只能检测少数几个靶标。我们开发了 ICEPlex 系统，采用可扩展的靶标分析程序（STAR）技术，以提供一种自动化、高多重PCR 解决方案。ICEPlex 将 PCR 热循环与毛细管电泳的动态、顺序扩增子分离以及双色定量检测相结合，集成在一个单一的系统中。与基于探针的 qPCR 不同，ICEPlex 通过掺入标记引物直接测量扩增子的积累。该系统展示了三个数量级的光学检测范围和至少 7 个数量级的可检测靶浓度范围。该系统可以在每个颜色通道中分离超过 50 个扩增子，大小从 100 到 500 个碱基，为广泛的核酸扩增应用提供了广泛的多重能力。ICEPlex 可用于分析病毒 DNA 或 RNA 靶标、检测遗传变异以及逆转录酶 PCR 基因表达面板。
