Nagase T, Ueno Y, Ishii S
Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Gene. 1990 Oct 15;94(2):249-53. doi: 10.1016/0378-1119(90)90395-8.
In the promoter region of the human c-Ha-ras1 gene, multiple elements for transcriptional control are dispersed. To examine the role of each element, we made a series of deletion mutants of this promoter region, including internal deletion mutants, and measured the promoter activities of these mutants. Any two of the four Sp1-binding sites upstream from the CCAAT-box element were sufficient for the maximal activity of the c-Ha-ras1 promoter. Either one of the two Sp1-binding sites downstream from the CACCC-box element was also necessary for the maximal promoter activity. Deletion of the CCAAT-box or CACCC-box elements reduced the level of transcription to 25% or 41% of that with the wild-type promoter. These results indicated that at least three Sp1-binding sites, and the CCAAT-box and CACCC-box elements contributed in concert to the activity of the human c-Ha-ras1 promoter.
在人类c-Ha-ras1基因的启动子区域，多个转录控制元件分散分布。为了研究每个元件的作用，我们构建了该启动子区域的一系列缺失突变体，包括内部缺失突变体，并检测了这些突变体的启动子活性。CCAAT框元件上游的四个Sp1结合位点中的任意两个对于c-Ha-ras1启动子的最大活性都是足够的。CACCC框元件下游的两个Sp1结合位点中的任意一个对于启动子的最大活性也是必需的。删除CCAAT框或CACCC框元件会使转录水平降至野生型启动子的25%或41%。这些结果表明，至少三个Sp1结合位点以及CCAAT框和CACCC框元件共同对人类c-Ha-ras1启动子的活性起作用。
