Ling B L, Dewaele C, Baeyens W R
Department of Pharmaceutical Chemistry and Drug Quality Control, State University of Ghent, Belgium.
J Chromatogr. 1990 Aug 29;514(2):189-98. doi: 10.1016/s0021-9673(01)89390-0.
Different thiol compounds of biological and pharmacological interest were separated on a packed reversed-phase fused-silica capillary column and determined with fluorescence detection. Conventional inexpensive liquid chromatographic equipment could be adapted for such purposes, providing a highly efficient analytical system. The different compounds were derivatized with the fluorogenic reagents SBD-F and ABD-F and chromatographed both isocratically and in the gradient mode in order to separate a series of thiols with widely varying polarities. Subsequently the derivatives were measured at lambda ex. = 380 nm and lambda em. = 510 nm. Application of the system to biological and pharmacological samples is suggested.
