使用红豆蔻凝集素通过高效液相亲和色谱法从人血清中纯化免疫球蛋白A

High-performance liquid affinity chromatography for the purification of immunoglobulin A from human serum using jacalin.

作者信息

Chui S H, Lam C W, Lewis W H, Lai K N

机构信息

Department of Health Sciences, Hong Kong Polytechnic, Kowloon.

出版信息

J Chromatogr. 1990 Aug 29;514(2):219-25. doi: 10.1016/s0021-9673(01)89393-6.

DOI:10.1016/s0021-9673(01)89393-6

PMID:2258396

Abstract

A high-performance liquid affinity chromatographic method for the purification of serum immunoglobulin A (IgA) using a jacalin column is described. The automated procedure takes about 2 with minimal manipulation. The yields of the isolated IgA and of its IgG and IgM contamination were studied by enzyme-linked immunosorbent assay (ELISA) of 30 sera. Purity was assured by immunoelectrophoresis. The ratio of IgA1 to total IgA was unchanged after purification, as verified by ELISA. The results showed that greater than 90% IgA could be recovered with less than 0.5% total IgG and greater than 2.0% total IgM remaining in the fractions containing purified IgA.

摘要

描述了一种使用红豆蔻凝集素柱纯化血清免疫球蛋白A（IgA）的高效液相亲和色谱方法。该自动化程序只需最少的操作，耗时约2小时。通过对30份血清进行酶联免疫吸附测定（ELISA），研究了分离出的IgA及其IgG和IgM污染物的产量。通过免疫电泳确保纯度。经ELISA验证，纯化后IgA1与总IgA的比例未变。结果表明，在含有纯化IgA的级分中，可回收超过90%的IgA，残留的总IgG不到0.5%，总IgM超过2.0%。