Department of Chemistry, Annamalai University, Annamalainagar, 608 002 Tamilnadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Sep;95:622-6. doi: 10.1016/j.saa.2012.04.064. Epub 2012 Apr 24.
The interaction between a trifluoromethyl substituted imidazole derivative 2-(4-(trifluoromethyl)phenyl)-1-phenyl-1H-imidazo[4,5-f] [1,10] phenanthroline (tfmppip) and bovine serum albumin (BSA) was investigated by solution spectral studies. The observed experimental result shows that the imidazole derivative has strong ability to quench the fluorescence of BSA by forming complex which is stabilized by electrostatic interactions. The effective quenching constants (k(sv)) were 2.79 × 10(4), 2.51 × 10(4), and 2.32 × 10(4) at 301, 310 and 318K respectively. The Stern-Volmer quenching constant (K(sv)), binding site number (n), apparent binding constant (K(A)) and corresponding thermodynamic parameters (ΔG, ΔH and ΔS) were calculated. The distance between the donor (BSA) and acceptor (tfmppip) was obtained according to fluorescence resonance energy transfer (FRET). Conformational changes of BSA were observed from synchronous fluorescence technique. The effect of metal ions such as Cu(2+), Zn(2+), Ca(2+), Mg(2+), Ni(2+), Co(2+) and Fe(2+) on the binding constants between the imidazole derivative and BSA were also studied.
通过溶液光谱研究了三氟甲基取代的咪唑衍生物 2-(4-(三氟甲基)苯基)-1-苯基-1H-咪唑[4,5-f][1,10]菲咯啉（tfmppip）与牛血清白蛋白（BSA）之间的相互作用。观察到的实验结果表明，咪唑衍生物通过形成复合物具有很强的能力来猝灭 BSA 的荧光，该复合物通过静电相互作用稳定。在 301、310 和 318K 时，有效猝灭常数（k(sv)）分别为 2.79×10(4)、2.51×10(4)和 2.32×10(4)。计算了 Stern-Volmer 猝灭常数（K(sv)）、结合位点数（n）、表观结合常数（K(A)）和相应的热力学参数（ΔG、ΔH 和 ΔS）。根据荧光共振能量转移（FRET）获得了供体（BSA）和受体（tfmppip）之间的距离。从同步荧光技术观察到 BSA 的构象变化。还研究了金属离子如 Cu(2+)、Zn(2+)、Ca(2+)、Mg(2+)、Ni(2+)、Co(2+)和 Fe(2+)对咪唑衍生物与 BSA 之间结合常数的影响。
