Buxbaum Engelbert
Ross University School of Medicine, Roseau, Commonwealth of Dominica, West Indies.
Methods Mol Biol. 2012;869:55-63. doi: 10.1007/978-1-61779-821-4_6.
Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist. However, there are situations where this method produces suboptimal results. In these cases, electrophoresis in the presence of positively charged detergents such as cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis and staining of such gels are presented.