[以[¹³C]/[¹²C]比率作为快速评估原油污染土壤中微生物群碳氢化合物氧化潜力的指标]

[Ratio [13C]/[12C] as an index for express estimation of hydrocarbon-oxidizing potential of microbiota in soil polluted with crude oil].

作者信息

Ziakun A M, Boronin A M, Kochetkov V V, Baskunov B P, Laurinavichius K S, Zakharchenko V N, Peshenko V P, Anokhina T O, Siunova T V

出版信息

Prikl Biokhim Mikrobiol. 2012 Mar-Apr;48(2):232-42.

PMID:22586918

Abstract

The hydrocarbon-oxidizing potential of soil microbiota and hydrocarbon-oxidizing microorganisms introduced into soil was studied based on the quantitative and isotopic characteristics of carbon in products formed in microbial degradation of oil hydrocarbons. Comparison of CO2 production rates in native soil and that polluted with crude oil showed the intensity of microbial mineralization of soil organic matter (SOM) in the presence of oil hydrocarbons to be higher as compared with non-polluted soil, that is, revealed a priming effect ofoil. The amount of carbon of newly synthesized organic products (cell biomass and exometabolites) due to consumed petroleum was shown to significantly exceed that of SOM consumed for production of CO2. The result of microbial processes in oil-polluted soil was found to be a potent release of carbon dioxide to the atmosphere.

摘要

基于石油烃微生物降解产物中碳的定量和同位素特征，研究了土壤微生物群和引入土壤的烃氧化微生物的烃氧化潜力。比较天然土壤和原油污染土壤中二氧化碳的产生速率，结果表明，与未污染土壤相比，在存在石油烃的情况下，土壤有机质（SOM）的微生物矿化强度更高，即显示出石油的激发效应。结果表明，由于消耗的石油而新合成的有机产物（细胞生物量和胞外代谢产物）中的碳量显著超过用于产生二氧化碳所消耗的SOM中的碳量。研究发现，石油污染土壤中微生物过程的结果是向大气中大量释放二氧化碳。