Tanaka H
Department of Pharmacology, Gunma University School of Medicine, Maebashi, Japan.
Neurosci Res Suppl. 1990;13:S147-51. doi: 10.1016/0921-8696(90)90043-3.
Selective axonal growth at a series of branching points along pathways is essential for the establishment of precise motoneuron projections. In order to reveal some of the molecules responsible for this selective axonal growth of motoneurons, I investigated the phenomenon of why motoneurons extend axons outside the spinal cord whereas interneurons do not. The analyses of immunohistology and embryonic surgery strongly suggested that motoneurons actively select to grow out from the cord and that interneurons also actively select to grow within the cord. The staining pattern of two monoclonal antibodies obtained from crude homogenate of young chick embryos were spatially and temporally correlated with motoneuron axonal growth. The antigenic molecules of these monoclonal antibodies were purified for the purpose of revealing their functions.
沿神经通路一系列分支点处的选择性轴突生长对于精确运动神经元投射的建立至关重要。为了揭示一些负责运动神经元这种选择性轴突生长的分子，我研究了运动神经元为何在脊髓外延伸轴突而中间神经元却不这样的现象。免疫组织学和胚胎手术分析强烈表明，运动神经元积极选择从脊髓中生长出来，而中间神经元也积极选择在脊髓内生长。从幼雏胚胎粗匀浆中获得的两种单克隆抗体的染色模式在空间和时间上与运动神经元轴突生长相关。为了揭示其功能，对这些单克隆抗体的抗原分子进行了纯化。
