采用两种不同的定量 HBsAg 检测方法检测体外表达的 HBsAg 突变体。
The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays.
机构信息
Institute of Virology, University of Cologne, Cologne, Germany.
出版信息
J Clin Virol. 2012 Jul;54(3):279-81. doi: 10.1016/j.jcv.2012.04.010. Epub 2012 May 16.
BACKGROUND
The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infected patients and to predict interferon treatment outcome.
OBJECTIVE
To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro.
STUDY DESIGN
HBsAg mutants (n=35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche).
RESULTS
All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system.
CONCLUSIONS
Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays.
背景
乙型肝炎表面抗原 (HBsAg) 的定量检测有助于在慢性 HBV 感染患者中识别无活性的病毒携带者，并预测干扰素治疗的效果。
目的
比较两种定量 HBsAg 检测方法，以检测体外表达的 HBsAg 突变体。
研究设计
在 HuH7 细胞中表达 HBsAg 突变体（n=35），并用两种不同的定量 HBsAg 检测方法（Architect，雅培和 Elecsys，罗氏）检测上清液。
结果
两种检测方法均能检测到所有 HBsAg 突变体，但总体而言，Architect 系统的结果高于 Elecsys 系统。两种检测方法对野生型和突变型 HBsAg 的检测相似。然而，Architect 系统对 HBsAg 突变体 T123A 的检测结果偏低，而 Elecsys 系统对 HBsAg 突变体 P142L、P142S 和 G145K 的检测结果偏低。
结论
尽管 HBsAg 检测方法已针对 HBsAg 突变体的检测进行了优化，但在两种定量 HBsAg 检测方法中，某些 HBsAg 突变体的检测结果存在差异。在使用两种不同的定量 HBsAg 检测方法检测同一个患者的样本时，需要考虑到这些发现。
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