Monté Boada R J, Pérez Rodríguez J M, Ramírez Alvarez M M, Dumas Valdieso S C
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
Rev Cubana Med Trop. 1990 Jan-Apr;42(1):13-8.
The presence of Yersinis enterocolitica was investigated in feces of 1,300 children younger than 5 years, hospitalized because of presenting acute diarrheic disease in Cuba during 1985-1988 period at different provincial pediatric hospital, as well as in Havana City. Selective agar base for Yersinia was used supplemented with cephsuloidin, Irgasan and novoviocin (CIN) and buffered saline solution as enrichment medium. This last one at 4 degrees C during 21 days with weekly resowings to the selective medium. Isolation of Yersinia enterocolitica was not obtained in any of the patients under study.
1985 - 1988年期间,在古巴不同省级儿童医院以及哈瓦那市,对1300名5岁以下因急性腹泻疾病住院的儿童粪便进行了小肠结肠炎耶尔森菌检测。使用添加了头孢磺啶、氯己定和新生霉素(CIN)的耶尔森菌选择性琼脂培养基,并使用缓冲盐溶液作为富集培养基。将缓冲盐溶液在4℃下保存21天,每周转接至选择性培养基。在所研究的任何患者中均未分离出小肠结肠炎耶尔森菌。