Borges A, Arantes E C, Giglio J R
Instituto Internacional de Estudios Avanzados, Caracas, Venezuela.
Toxicon. 1990;28(9):1011-7. doi: 10.1016/0041-0101(90)90139-x.
Four toxic, electrophoretically homogeneous proteins were isolated by ion-exchange chromatography on CM-cellulose-52 from the venom of the scorpion Tityus discrepans (range North Central Venezuela), named TdIV, TdV, TdVIII and TdIX. Component TdVIII, with 56 amino acid residues and mol. wt 6140 was the most toxic by i.p. injections into mice and had an intracisternal LD50 of 7.9 micrograms protein/kg body weight. Amino acid compositions of components TdIV and TdV were very similar, suggesting that they could be highly homologous proteins, although presumably contaminated one by the other. A fifth component, named TdIII, non-toxic by i.p. injections, was also isolated in homogeneous form. The i.v. and intracisternal LD50 values of the whole T. discrepans venom were 2.5 mg/kg and 16.0 micrograms/kg, respectively.
通过在CM - 纤维素 - 52上进行离子交换色谱法，从委内瑞拉中北部的差异肥尾蝎毒液中分离出四种有毒的、电泳均一的蛋白质，分别命名为TdIV、TdV、TdVIII和TdIX。组分TdVIII含有56个氨基酸残基，分子量为6140，通过腹腔注射给小鼠时毒性最强，脑池内注射的半数致死量为7.9微克蛋白质/千克体重。组分TdIV和TdV的氨基酸组成非常相似，表明它们可能是高度同源的蛋白质，尽管可能相互污染。还以均一形式分离出了第五种组分，命名为TdIII，腹腔注射无毒。差异肥尾蝎全毒液的静脉注射和脑池内注射半数致死量分别为2.5毫克/千克和16.0微克/千克。
